Human Interleukin 1 Receptor Accessory Protein (IL1RAP) ELISA Kit | IL1RAP elisa kit

Human Interleukin 1 Receptor Accessory Protein (IL1RAP) ELISA Kit

Cat. Num. AAA451664

• Gene Names: IL1RAP; IL1R3; C3orf13; IL-1RAcP
• Reactivity: Human
• Synonyms: Interleukin 1 Receptor Accessory Protein (IL1RAP); Human Interleukin 1 Receptor Accessory Protein (IL1RAP) ELISA Kit; IL-1RAcP; C3orf13; IL1R3; Interleukin-1 receptor 3; IL1RAP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of IL1RAP. No significant cross-reactivity or interference between IL1RAP and analogues was observed.
• Sequence Length: 570

• Samples: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.065ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IL1RAP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IL1RAP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL1RAP elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL1RAP in human serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL1RAP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to IL1RAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL1RAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IL1RAP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 268840214
• NCBI GeneID: 3556
• NCBI Accession #: NP_001161400.1
• NCBI GenBank Nucleotide #: NM_001167928.1
• UniProt Accession #: Q9NPH3; O14915; Q86WJ7; B1NLD0; D3DNW0
• Molecular Weight: 78,603 Da
• NCBI Official Full Name: interleukin-1 receptor accessory protein isoform 1
• NCBI Official Synonym Full Names: interleukin 1 receptor accessory protein
• NCBI Official Symbol: IL1RAP
• NCBI Official Synonym Symbols: IL1R3; C3orf13; IL-1RAcP
• NCBI Protein Information: interleukin-1 receptor accessory protein
• UniProt Protein Name: Interleukin-1 receptor accessory protein
• Protein Family: Interleukin-1 receptor accessory protein
• UniProt Gene Name: IL1RAP
• UniProt Synonym Gene Names: C3orf13; IL1R3; IL-1 receptor accessory protein; IL-1RAcP; IL-1R-3; IL-1R3

NCBI Description

Interleukin 1 induces synthesis of acute phase and proinflammatory proteins during infection, tissue damage, or stress, by forming a complex at the cell membrane with an interleukin 1 receptor and an accessory protein. This gene encodes the interleukin 1 receptor accessory protein. The protein is a necessary part of the interleukin 1 receptor complex which initiates signalling events that result in the activation of interleukin 1-responsive genes. Alternative splicing of this gene results in two transcript variants encoding two different isoforms, one membrane-bound and one soluble. The ratio of soluble to membrane-bound forms increases during acute-phase induction or stress. [provided by RefSeq, Nov 2009]

Uniprot Description

Coreceptor for IL1RL2 in the IL-36 signaling system (). Coreceptor with IL1R1 in the IL-1 signaling system. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Recruits TOLLIP to the signaling complex. Does not bind to interleukin-1 alone; binding of IL1RN to IL1R1, prevents its association with IL1R1 to form a signaling complex. The cellular response is modulated through a non-signaling association with the membrane IL1R2 decoy receptor. Coreceptor for IL1RL1 in the IL-33 signaling system.