Human Integrin Alpha 11 (ITGa11) ELISA Kit | ITGa11 elisa kit

Human Integrin Alpha 11 (ITGa11) ELISA Kit

Cat. Num. AAA2023245

• Gene Names: ITGA11; HsT18964
• Reactivity: Human
• Synonyms: Integrin Alpha 11 (ITGa11); Human Integrin Alpha 11 (ITGa11) ELISA Kit; ITG-A11; ITGa11 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Integrin Alpha 11 (ITGa11).; No significant cross-reactivity or interference between Integrin Alpha 11 (ITGa11) and analogues was observed.
• Sequence Length: 1,189

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.124ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Integrin Alpha 11 (ITGa11) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Integrin Alpha 11 (ITGa11) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ITGa11 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Alpha 11 (ITGa11). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Integrin Alpha 11 (ITGa11). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Integrin Alpha 11 (ITGa11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Integrin Alpha 11 (ITGa11) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for ITGa11 elisa kit

CD & Adhesion molecule; Tumor immunity; Bone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 52485853
• NCBI GeneID: 22801
• NCBI Accession #: NP_001004439.1
• NCBI GenBank Nucleotide #: NM_001004439.1
• UniProt Accession #: Q9UKX5; Q8WYI8; Q9UKQ1; J3KQM2
• Molecular Weight: 133,569 Da
• NCBI Official Full Name: integrin alpha-11
• NCBI Official Synonym Full Names: integrin, alpha 11
• NCBI Official Symbol: ITGA11
• NCBI Official Synonym Symbols: HsT18964
• NCBI Protein Information: integrin alpha-11
• UniProt Protein Name: Integrin alpha-11
• Protein Family: Integrin
• UniProt Gene Name: ITGA11
• UniProt Entry Name: ITA11_HUMAN

NCBI Description

This gene encodes an alpha integrin. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein contains an I domain, is expressed in muscle tissue, dimerizes with beta 1 integrin in vitro, and appears to bind collagen in this form. Therefore, the protein may be involved in attaching muscle tissue to the extracellular matrix. Alternative transcriptional splice variants have been found for this gene, but their biological validity is not determined. [provided by RefSeq, Jul 2008]

Uniprot Description

ITGA11: Integrin alpha-11/beta-1 is a receptor for collagen. Belongs to the integrin alpha chain family.

Protein type: Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 15q23

Cellular Component: focal adhesion; membrane; plasma membrane; integrin complex

Molecular Function: collagen binding; metal ion binding

Biological Process: osteoblast differentiation; substrate-bound cell migration; integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; muscle development; cell-matrix adhesion; cell adhesion mediated by integrin; cell adhesion