Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Inositol oxygenase (MIOX) Standard Curve.)
Human Inositol Oxygenase (MIOX) ELISA Kit | MIOX elisa kit
Human Inositol Oxygenase (MIOX) ELISA Kit
Gene Names
MIOX; ALDRL6
Reactivity
Human
Synonyms
Inositol Oxygenase (MIOX); Human Inositol Oxygenase (MIOX) ELISA Kit; MIOX; RP4-579N16.11; ALDRL6; MGC90217; aldehyde reductase (aldose reductase) like 6; kidney-specific protein 32; MIOX elisa kit
Reactivity
Human
Specificity
Human Inositol oxygenase (MIOX) ELISA Kit has high sensitivity and excellent specificity for detection of Human MIOX. No significant cross-reactivity or interference between Human MIOX and analogues was observed.
Sequence Length
285
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Inositol oxygenase (MIOX) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Inositol oxygenase (MIOX) Standard Curve.)
Related Product Information for MIOX elisa kit
Description: This Human Inositol oxygenase (MIOX) ELISA Kit employs a two-site sandwich ELISA to quantitate MIOX in samples. An antibody specific for MIOX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MIOX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MIOX is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MIOX bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MIOX (Myo-Inositol Oxygenase) is a Protein Coding gene. Among its related pathways are Inositol phosphate metabolism (KEGG) and Porphyrin and chlorophyll metabolism. GO annotations related to this gene include iron ion binding and oxidoreductase activity, acting on NAD(P)H.
Background: MIOX (Myo-Inositol Oxygenase) is a Protein Coding gene. Among its related pathways are Inositol phosphate metabolism (KEGG) and Porphyrin and chlorophyll metabolism. GO annotations related to this gene include iron ion binding and oxidoreductase activity, acting on NAD(P)H.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
inositol oxygenase
NCBI Official Synonym Full Names
myo-inositol oxygenase
NCBI Official Symbol
MIOX
NCBI Official Synonym Symbols
ALDRL6
NCBI Protein Information
inositol oxygenase
UniProt Protein Name
Inositol oxygenase
Protein Family
UniProt Gene Name
MIOX
UniProt Synonym Gene Names
ALDRL6; KSP32; RSOR; MI oxygenase
UniProt Entry Name
MIOX_HUMAN
Uniprot Description
MIOX: Belongs to the myo-inositol oxygenase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Carbohydrate Metabolism - ascorbate and aldarate; Carbohydrate Metabolism - inositol phosphate; EC 1.13.99.1; Oxidoreductase
Chromosomal Location of Human Ortholog: 22q13.3
Cellular Component: cytoplasm; inclusion body
Molecular Function: aldo-keto reductase activity; ferric iron binding; inositol oxygenase activity; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen
Biological Process: inositol catabolic process
Research Articles on MIOX
Similar Products
Product Notes
The Human MIOX miox (Catalog #AAA9715273) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715273 ELISA Kit recognizes Human MIOX. It is sometimes possible for the material contained within the vial of "Inositol Oxygenase (MIOX), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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