Human Inhibin (INH) ELISA Kit | INH elisa kit

Human Inhibin (INH) ELISA Kit

Cat. Num. AAA265890

• Reactivity: Human
• Synonyms: Inhibin (INH); Human Inhibin (INH) ELISA Kit; INH elisa kit

• Reactivity: Human
• Sequence Length: 407

• Samples: Human serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 2000 pg/mL-31.2 pg/mL
• Sensitivity: Up to 12 pg/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for INH elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human INH monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 154813204
• NCBI GeneID: 3625
• NCBI Accession #: NP_002184.2
• NCBI GenBank Nucleotide #: NM_002193.2
• Molecular Weight: 45,122 Da
• NCBI Official Full Name: inhibin beta B chain preproprotein
• NCBI Official Synonym Full Names: inhibin, beta B
• NCBI Official Symbol: INHBB
• NCBI Protein Information: inhibin beta B chain; Inhibin, beta-2; activin beta-B chain; activin AB beta polypeptide
• UniProt Protein Name: Inhibin beta B chain
• Protein Family: Protein
• UniProt Gene Name: INHBB
• UniProt Entry Name: INHBB_HUMAN

NCBI Description

The inhibin beta B subunit joins the alpha subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumour-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. Because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone. Furthermore, the beta B subunit forms a homodimer, activin B, and also joins with the beta A subunit to form a heterodimer, activin AB, both of which stimulate FSH secretion. [provided by RefSeq, Jul 2008]

Uniprot Description

INHBB: Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. Belongs to the TGF-beta family.

Protein type: Secreted, signal peptide; Cytokine; Cell development/differentiation; Secreted

Chromosomal Location of Human Ortholog: 2cen-q13

Cellular Component: extracellular space; perinuclear region of cytoplasm; extracellular region

Molecular Function: protein binding; protein homodimerization activity; growth factor activity; hormone activity; cytokine activity; host cell surface receptor binding; transforming growth factor beta receptor binding

Biological Process: fat cell differentiation; activin receptor signaling pathway; defense response; negative regulation of hepatocyte growth factor biosynthetic process; cellular response to starvation; negative regulation of follicle-stimulating hormone secretion; negative regulation of insulin secretion; regulation of apoptosis; cellular response to insulin stimulus; ovarian follicle development; response to mechanical stimulus; regulation of MAPKKK cascade; positive regulation of follicle-stimulating hormone secretion; cell differentiation; cell development; oocyte development; growth