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IL-6 elisa kit

Human IL-6 ELISA Kit

Gene Names
Il6; ILg6; Ifnb2
Reactivity
Human. No cross reactivity with other human cytokines. No interference with soluble receptor gp80.
Synonyms
IL-6; Human IL-6 ELISA Kit; IL-6 elisa kit
Ordering
For Research Use Only!
Reactivity
Human. No cross reactivity with other human cytokines. No interference with soluble receptor gp80.
Specificity
Recognizes both natural and recombinant human IL-6
Sequence Length
211
Sensitivity
The sensitivity, minimum detectable dose of IL-6 using this IL-6 ELISA kit was found to be less than 2pg/ml. This was determined by adding 3 standard deviations to the mean OD obtained when the zero standard was assayed 40 times.
Intra-assay Precision
Reproductibility within the assay was evaluated in three independent experiments by two different analysts. Each assay was carried out with 6 replicates (3 duplicates) of 3 spiked human pooled serum samples and 2 supernatants containing different concentrations of IL-6. Data below shows the mean IL-6 concentration and the coefficient of variation for each sample. The overall intra-assay coefficient of variation has been calculated to be 4.2%.
Inter-assay Precision
Assay to assay reproductibility within one laboratory was evaluated in three independent experiments by two analysts. Each assay was carried out with 6 replicates of 3 spiked serum human pooled serum samples and 2 supernatants containing different concentrations of IL-6. The calculated overall coefficient of variation was 7.7%
Related Product Information for IL-6 elisa kit
MyBioSource ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents, and a detailed procedure.
Product Categories/Family for IL-6 elisa kit
References
Auf, G. et al., PNAS,2010;0914072107
Azadbakht, L. et al., Diabetes Care,2007;30(4):967-73
Badoual, C. et al., Cancer Res.,2008; 68(10): 3907-3914.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24,357 Da
NCBI Official Full Name
interleukin-6
NCBI Official Synonym Full Names
interleukin 6
NCBI Official Symbol
Il6
NCBI Official Synonym Symbols
ILg6; Ifnb2
NCBI Protein Information
interleukin-6
UniProt Protein Name
Interleukin-6
UniProt Gene Name
Il6
UniProt Synonym Gene Names
Il-6; IL-6
UniProt Entry Name
IL6_RAT

NCBI Description

a cytokine involved in development and possibly in neurodegenerative processes [RGD, Feb 2006]

Uniprot Description

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation ().

Research Articles on IL-6

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Product Notes

The Human. No cross reactivity with other human cytokines. No interference with soluble receptor gp80. IL-6 il6 (Catalog #AAA335510) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA335510 ELISA Kit recognizes Human. No cross reactivity with other human cytokines. No interference with soluble receptor gp80. IL-6. It is sometimes possible for the material contained within the vial of "IL-6, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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