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Typical Testing Data/Standard Curve (for reference only)

Human IL-6 (Interleukin 6) ELISA Kit | IL-6 elisa kit

QuicKey Pro Human IL-6 (Interleukin 6) ELISA Kit

Gene Names
IL6; HGF; HSF; BSF2; IL-6; IFNB2
Reactivity
Human
Synonyms
IL-6 (Interleukin 6); QuicKey Pro Human IL-6 (Interleukin 6) ELISA Kit; Interleukin 6; IL6; BSF2; HGF; HSF; IFNB2; CDF; IL-6; IL-6 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This kit recognizes IL-6 in samples. No significant cross-reactivity or interference between IL-6 and analogues was observed.
This ELISA kit applies to the in vitro quantitative determination of IL-6 concentrations in serum and plasma.
Assay Type
Sandwich ELISA
Detection Method
Colormetric
Tested Sample Types
Serum and plasma
Detection Range
3.13-200pg/mL
Sensitivity
1.02pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-6. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human IL-6 are added to the micro ELISA plate wells. Human IL-6 in samples (or standards) combines with the coated antibody and HRP linked detection antibody specific to IL-6. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450+/-2nm. The concentration of Human IL-6 in the samples is then determined by comparing the OD of the samples to the standard curve.
Preparation and Storage
An unopened kit can be stored at 2-8? for 6 months. After test, the unused wells and reagents should be stored according to the table/manual.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
212
NCBI Official Full Name
Interleukin-6
NCBI Official Synonym Full Names
interleukin 6
NCBI Official Symbol
IL6
NCBI Official Synonym Symbols
HGF; HSF; BSF2; IL-6; IFNB2
NCBI Protein Information
interleukin-6; CDF; BSF-2; IFN-beta-2; interferon beta-2; interleukin BSF-2; interferon, beta 2; hybridoma growth factor; CTL differentiation factor; B-cell stimulatory factor 2; B-cell differentiation factor
UniProt Protein Name
Interleukin-6
UniProt Gene Name
IL6
UniProt Synonym Gene Names
IFNB2; IL-6; BSF-2; CDF; IFN-beta-2
UniProt Entry Name
IL6_HUMAN

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Product Notes

The Human IL-6 il6 (Catalog #AAA2568085) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2568085 ELISA Kit recognizes Human IL-6. It is sometimes possible for the material contained within the vial of "IL-6 (Interleukin 6), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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