Human IL-17RA PicoKine ELISA Kit | IL17RA elisa kit

Human IL-17RA PicoKine ELISA Kit

Cat. Num. AAA177575

• Gene Names: IL17RA; CD217; IL17R; CANDF5; CDw217; IL-17RA; hIL-17R
• Reactivity: Human
• Synonyms: IL-17RA PicoKine; Human IL-17RA PicoKine ELISA Kit; Interleukin-17 receptor A; CANDF5; CD217; CD217 antigen; CDw217; CTLA8; HIL 17R; I17RA_HUMAN; IL 17 receptor A; IL 17 receptor; IL 17RA; IL-17 receptor A; IL-17RA; IL17; IL17A receptor; IL17R; IL17RA; Interleukin 17 receptor A; MGC10262 ; interleukin 17 receptor A; IL17RA elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human IL-17RA
• Sequence Length: 832

• Assay Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <10pg/ml
• Sample: For quantitative detection of human IL-17RA in cell culture supernates, serum and plasma(heparin, EDTA).
• Immunogen: NSO, L33-W320
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml human IL-17RA standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum and plasma (heparin, EDTA) to each empty well. It is recommended that each human IL-17RA standard solution and each sample be measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml)|258|762|1229|262|770|1335; Standard deviation|17.02|44.95|70.05|18.34|49.28|82.77; CV(%)|6.6|5.9|5.7|7.0|6.4|6.2
• Sample Data: Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000; O.D.|0.033|0.108|0.182|0.287|0.531|0.912|1.613|2.116
• Anticoagulant: Heparin or EDTA
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Human IL-17RA PicoKine ELISA Kit standard curve )

Related Product Information for IL17RA elisa kit

Principle of the assay: Human IL-17RA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-17RA has been precoated onto 96-well plates. Standards (NSO, L33- W320) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17RA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-17RA amount of sample captured in plate.

Background: Interleukin 17 receptor A, also known as IL17RA and CDw217 (cluster of differentiation w217), is a human gene. The IL17R gene is mapped to 22q11.22-q11.23 by radiation hybrid analysis. Interleukin 17A (IL17A) is a proinflammatory cytokine secreted by activated T-lymphocytes. It is a potent inducer of the maturation of CD34-positive hematopoietic precursors into neutrophils. The protein encoded by this gene is a ubiquitous type I membrane glycoprotein that binds with low affinity to interleukin 17A. Interleukin 17A and its receptor play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis. Like other cytokine receptors, this receptor likely has a multimeric structure. Also, it is showed that mouse Il17r bound HSV-13 and mouse Il17. Recombinant soluble Il17r inhibited mitogen-induced T-cell proliferation and Il2 production.

NCBI and Uniprot Product Information

• NCBI GI #: 583966138
• NCBI GeneID: 23765
• NCBI Accession #: NP_001276834.1
• NCBI GenBank Nucleotide #: NM_001289905.1
• UniProt Accession #: Q96F46; O43844; Q20WK1
• Molecular Weight: 92,153 Da
• NCBI Official Full Name: interleukin-17 receptor A isoform 2
• NCBI Official Synonym Full Names: interleukin 17 receptor A
• NCBI Official Symbol: IL17RA
• NCBI Official Synonym Symbols: CD217; IL17R; CANDF5; CDw217; IL-17RA; hIL-17R
• NCBI Protein Information: interleukin-17 receptor A
• UniProt Protein Name: Interleukin-17 receptor A
• Protein Family: Interleukin-17 receptor
• UniProt Gene Name: IL17RA
• UniProt Synonym Gene Names: IL17R; IL-17 receptor A; IL-17RA
• UniProt Entry Name: I17RA_HUMAN

NCBI Description

Interleukin 17A (IL17A) is a proinflammatory cytokine secreted by activated T-lymphocytes. It is a potent inducer of the maturation of CD34-positive hematopoietic precursors into neutrophils. The transmembrane protein encoded by this gene (interleukin 17A receptor; IL17RA) is a ubiquitous type I membrane glycoprotein that binds with low affinity to interleukin 17A. Interleukin 17A and its receptor play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis. Like other cytokine receptors, this receptor likely has a multimeric structure. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2014]

Uniprot Description

IL17RA: Receptor for IL17A and IL17F. Binds its IL17A ligand with low affinity, suggesting that additional components are involved in IL17A-induced signaling. Widely expressed.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 22q11.1

Cellular Component: extracellular region; integral to plasma membrane; plasma membrane

Molecular Function: interleukin-17 receptor activity; protein binding; receptor binding

Biological Process: cell surface receptor linked signal transduction; cytokine and chemokine mediated signaling pathway; defense response to fungus; positive regulation of interleukin-23 production

Disease: Candidiasis, Familial, 5