Human IL-12 p35 ELISA Kit | IL-12 p35 elisa kit

Human IL-12 p35 (Interleukin 12 p35) ELISA Kit

Cat. Num. AAA2515711

• Gene Names: IL12A; P35; CLMF; NFSK; NKSF1; IL-12A
• Reactivity: Human
• Synonyms: IL-12 p35; Human IL-12 p35 (Interleukin 12 p35) ELISA Kit; IL-12 p35 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes Human IL-12 p35 in samples. No significant cross-reactivity or interference between Human IL-12 p35 and analogues was observed.
• Sequence Length: 253

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 7.81-500pg/mL
• Sensitivity: 4.69pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-12 p35 were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-12 p35 were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-12 p35 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human IL-12 p35 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-12 p35. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-12 p35 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-12 p35, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human IL-12 p35. You can calculate the concentration of Human IL-12 p35 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 24430219
• NCBI GeneID: 3592
• NCBI Accession #: NP_000873.2
• NCBI GenBank Nucleotide #: NM_000882.3
• Molecular Weight: 24,874 Da
• NCBI Official Full Name: interleukin-12 subunit alpha
• NCBI Official Synonym Full Names: interleukin 12A
• NCBI Official Symbol: IL12A
• NCBI Official Synonym Symbols: P35; CLMF; NFSK; NKSF1; IL-12A
• NCBI Protein Information: interleukin-12 subunit alpha; CLMF p35; IL-12, subunit p35; IL35 subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; cytotoxic lymphocyte maturation factor 1, p35; cytotoxic lymphocyte maturation factor 35 kDa subunit; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin-12 alpha chain
• UniProt Protein Name: Interleukin-12 subunit alpha
• UniProt Gene Name: IL12A
• UniProt Synonym Gene Names: NKSF1; IL-12A; CLMF p35; NKSF1
• UniProt Entry Name: IL12A_HUMAN

NCBI Description

This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]

Uniprot Description

IL12A: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine- activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. Belongs to the IL-6 superfamily.

Protein type: Secreted; Cytokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3q25.33

Cellular Component: extracellular space; interleukin-12 complex; cytoplasm

Molecular Function: interleukin-27 binding; protein binding; interleukin-12 beta subunit binding; growth factor activity; protein heterodimerization activity; cytokine activity; interleukin-12 receptor binding

Biological Process: cell migration; positive regulation of NK T cell activation; positive regulation of cell adhesion; positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target; negative regulation of smooth muscle cell proliferation; positive regulation of T cell mediated cytotoxicity; response to virus; positive regulation of tyrosine phosphorylation of Stat4 protein; response to lipopolysaccharide; positive regulation of natural killer cell mediated cytotoxicity; defense response to protozoan; positive regulation of natural killer cell activation; response to UV-B; defense response to Gram-positive bacterium; positive regulation of interferon-gamma production; negative regulation of interleukin-17 production; positive regulation of mononuclear cell proliferation; positive regulation of T cell differentiation; positive regulation of lymphocyte proliferation; positive regulation of T cell proliferation; immune response; cell cycle arrest