Human IgM ELISA Kit | IgM elisa kit

Human IgM ELISA Kit

Cat. Num. AAA9135808

• Gene Names: IGHM; MU; VH; AGM1
• Reactivity: Human
• Synonyms: IgM; Human IgM ELISA Kit; IgM elisa kit

• Reactivity: Human
• Specificity: This assay recognizes both recombinant and natural human IgM. The factors listed below were prepared at 50ng/ml and assayed for cross-reactivity.
• Sequence Length: 112

• Assay Type: Quantitative Sandwich
• Sensitivity: < 1.56ng/mL
• Intra-assay Precision: Intra-plate Precision The three samples with known concentrations were repeated 20 times on the same board to calculate the coefficient of variation (CV) for the concentrations. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-plate Precision The coefficient of variation (CV) of three known concentrations was calculated by 20 tests of two kits on each of the three samples. Inter-Assay: CV<15% Inter-Assay: CV<15%
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IgM elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for human IgM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgM present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-conjugated detection antibody specific for human IgM is added to the wells. After incubating, an enzyme-linked streptavidin is added and binds to biotin. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IgM bound in the initial step. The color development is stopped, and the absorbance is measured.

Background/Introduction: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IgM in human serum, plasma, cell culture supernatants and other biological fluids.

NCBI and Uniprot Product Information

• NCBI GI #: 473215
• NCBI GeneID: 3507
• Molecular Weight: 51,924 Da
• NCBI Official Full Name: IgM, partial
• NCBI Official Synonym Full Names: immunoglobulin heavy constant mu
• NCBI Official Symbol: IGHM
• NCBI Official Synonym Symbols: MU; VH; AGM1
• UniProt Protein Name: Immunoglobulin heavy constant mu
• Protein Family: IgM protease
• UniProt Gene Name: IGHM

NCBI Description

Immunoglobulins (Ig) are the antigen recognition molecules of B cells. An Ig molecule is made up of 2 identical heavy chains and 2 identical light chains (see MIM 147200) joined by disulfide bonds so that each heavy chain is linked to a light chain and the 2 heavy chains are linked together. Each Ig heavy chain has an N-terminal variable (V) region containing the antigen-binding site and a C-terminal constant (C) region, encoded by an individual C region gene, that determines the isotype of the antibody and provides effector or signaling functions. The heavy chain V region is encoded by 1 each of 3 types of genes: V genes (see MIM 147070), joining (J) genes (see MIM 147010), and diversity (D) genes (see MIM 146910). The C region genes are clustered downstream of the V region genes within the heavy chain locus on chromosome 14. The IGHM gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD (see IGHD; MIM 1471770) on their surface. During an antibody response, activated B cells can switch to the expression of individual downstream heavy chain C region genes by a process of somatic recombination known as isotype switching. In addition, secreted Ig forms that act as antibodies can be produced by alternative RNA processing of the heavy chain C region sequences. Although the membrane forms of all Ig isotypes are monomeric, secreted IgM forms pentamers, and occasionally hexamers, in plasma (summary by Janeway et al., 2005).[supplied by OMIM, Aug 2010]

Uniprot Description

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268). IgM antibodies play an important role in primary defense mechanisms. They have been shown to be involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions. The membrane-bound form is found in the majority of normal B-cells alongside with IgD. Membrane-bound IgM induces the phosphorylation of CD79A and CD79B by the Src family of protein tyrosine kinases. It may cause death of cells by apoptosis. It is also found in soluble form, which represents about 30% of the total serum immunoglobulins where it is found almost exclusively as a homopentamer. After the antigen binds to the B-cell receptor, the secreted form is secreted in large amounts (PubMed:3137579, PubMed:16895553).