Typical Testing Data/Standard Curve (for reference only)
Human Hypoxanthine guanine phosphoribosyltransferase (HPRT1) ELISA Kit | HPRT1 elisa kit
Human Hypoxanthine guanine phosphoribosyltransferase (HPRT1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: HPRT1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-HPRT1 antibody and an HPRT1-HRP conjugate. The assay sample and buffer are incubated together with HPRT1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HPRT1 concentration since HPRT1 from samples and HPRT1-HRP conjugate compete for the anti-HPRT1 antibody binding site. Since the number of sites is limited, as more sites are occupied by HPRT1 from the sample, fewer sites are left to bind HPRT1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HPRT1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.[provided by RefSeq, Jun 2009]
Uniprot Description
HPRT1: Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5- phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway. Defects in HPRT1 are the cause of Lesch-Nyhan syndrome (LNS). LNS is characterized by complete lack of enzymatic activity that results in hyperuricemia, choreoathetosis, mental retardation, and compulsive self-mutilation. Defects in HPRT1 are the cause of gout HPRT-related (GOUT-HPRT); also known as HPRT-related gout or Kelley-Seegmiller syndrome. Gout is characterized by partial enzyme activity and hyperuricemia. Belongs to the purine/pyrimidine phosphoribosyltransferase family.
Protein type: Cell development/differentiation; Xenobiotic Metabolism - drug metabolism - other enzymes; Nucleotide Metabolism - purine; EC 2.4.2.8; Transferase
Chromosomal Location of Human Ortholog: Xq26.1
Cellular Component: cytoplasm; cytosol
Molecular Function: protein binding; protein homodimerization activity; magnesium ion binding; hypoxanthine phosphoribosyltransferase activity; nucleotide binding
Biological Process: hypoxanthine metabolic process; IMP salvage; cytolysis; locomotory behavior; adenine salvage; dopamine metabolic process; purine salvage; protein homotetramerization; cerebral cortex neuron differentiation; IMP metabolic process; hypoxanthine salvage; central nervous system neuron development; grooming behavior; lymphocyte proliferation; striatum development; GMP salvage; nucleobase, nucleoside and nucleotide metabolic process; dendrite morphogenesis; response to amphetamine; purine nucleotide biosynthetic process; purine base metabolic process; GMP catabolic process; positive regulation of dopamine metabolic process; purine ribonucleoside salvage; guanine salvage
Disease: Kelley-seegmiller Syndrome; Lesch-nyhan Syndrome
Research Articles on HPRT1
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Product Notes
The Human HPRT1 hprt1 (Catalog #AAA7201499) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7201499 ELISA Kit recognizes Human HPRT1. It is sometimes possible for the material contained within the vial of "Hypoxanthine guanine phosphoribosyltransferase (HPRT1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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