Human Huntingtin Interacting Protein 1 (HIP1) ELISA Kit | HIP1 elisa kit

Human Huntingtin Interacting Protein 1 (HIP1) ELISA Kit

Cat. Num. AAA2122050

• Gene Names: HIP1; SHON; HIP-I; ILWEQ; SHONbeta; SHONgamma
• Reactivity: Human
• Synonyms: Huntingtin Interacting Protein 1 (HIP1); Human Huntingtin Interacting Protein 1 (HIP1) ELISA Kit; ILWEQ; HIP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Huntingtin Interacting Protein 1 (HIP1).; No significant cross-reactivity or interference between Huntingtin Interacting Protein 1 (HIP1) and analogues was observed.
• Sequence Length: 1037

• Assay Type: Double-antibody Sandwich
• Sample: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Dectection Range: 0.312-20ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.129ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Huntingtin Interacting Protein 1 (HIP1) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Huntingtin Interacting Protein 1 (HIP1) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HIP1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Huntingtin Interacting Protein 1 (HIP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Huntingtin Interacting Protein 1 (HIP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Huntingtin Interacting Protein 1 (HIP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Huntingtin Interacting Protein 1 (HIP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for HIP1 elisa kit

Immune Molecule

NCBI and Uniprot Product Information

• NCBI GI #: 206729937
• NCBI GeneID: 3092
• UniProt Accession #: O00291
• NCBI Official Full Name: Huntingtin-interacting protein 1
• NCBI Official Synonym Full Names: huntingtin interacting protein 1
• NCBI Official Symbol: HIP1
• NCBI Official Synonym Symbols: SHON; HIP-I; ILWEQ; SHONbeta; SHONgamma
• NCBI Protein Information: huntingtin-interacting protein 1
• UniProt Protein Name: Huntingtin-interacting protein 1
• Protein Family: Histidine permease
• UniProt Gene Name: HIP1
• UniProt Synonym Gene Names: HIP-1; HIP-I
• UniProt Entry Name: HIP1_HUMAN

NCBI Description

The product of this gene is a membrane-associated protein that functions in clathrin-mediated endocytosis and protein trafficking within the cell. The encoded protein binds to the huntingtin protein in the brain; this interaction is lost in Huntington's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

HIP1: Plays a role in clathrin-mediated endocytosis and trafficking. Involved in regulating AMPA receptor trafficking in the central nervous system in an NMDA-dependent manner. Enhances androgen receptor (AR)-mediated transcription. May act as a proapoptotic protein that induces cell death by acting through the intrinsic apoptosis pathway. Binds 3-phosphoinositides (via ENTH domain). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis. May play a functional role in the cell filament networks. May be required for differentiation, proliferation, and/or survival of somatic and germline progenitors. A chromosomal aberration involving HIP1 is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;7)(q33;q11.2) with PDGFRB. The chimeric HIP1-PDGFRB transcript results from an in-frame fusion of the two genes. The reciprocal PDGFRB-HIP1 transcript is not expressed. Belongs to the SLA2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal; Apoptosis

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: Golgi apparatus; cytoskeleton; intracellular membrane-bound organelle; clathrin-coated vesicle; membrane; cytoplasm; clathrin coated vesicle membrane; nucleus

Molecular Function: protein binding, bridging; clathrin binding; protein binding; structural constituent of cytoskeleton; actin binding; phosphoinositide binding

Biological Process: regulation of apoptosis; caspase activation; clathrin cage assembly; transcription, DNA-dependent; regulation of transcription, DNA-dependent; apoptosis; endocytosis; cell differentiation; actin cytoskeleton organization and biogenesis; positive regulation of receptor-mediated endocytosis

Disease: Prostate Cancer