Human Histone H4-like protein type G (HIST1H4G) ELISA Kit | HIST1H4G elisa kit

Human Histone H4-like protein type G (HIST1H4G) ELISA Kit

Cat. Num. AAA2800090

• Gene Names: HIST1H4G; H4/l; H4FL
• Reactivity: Human
• Synonyms: Histone H4-like protein type G (HIST1H4G); Human Histone H4-like protein type G (HIST1H4G) ELISA Kit; H4/l; H4FL; H4 histone family; member L; histone 1; H4g; HIST1H4G elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human HIST1H4G. No significant cross-reactivity or interference between Human HIST1H4G and analogues was observed.
• Sequence Length: 98

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for HIST1H4G elisa kit

This assay employs a two-site sandwich ELISA to quantitate HIST1H4G in samples. An antibody specific for HIST1H4G has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HIST1H4G present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HIST1H4G is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HIST1H4G bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4504319
• NCBI GeneID: 8369
• NCBI Accession #: NP_003538.1
• NCBI GenBank Nucleotide #: NM_003547.2
• UniProt Accession #: Q99525
• Molecular Weight: 11,009 Da
• NCBI Official Full Name: histone H4-like protein type G
• NCBI Official Synonym Full Names: histone cluster 1 H4 family member g
• NCBI Official Symbol: HIST1H4G
• NCBI Official Synonym Symbols: H4/l; H4FL
• NCBI Protein Information: histone H4-like protein type G
• UniProt Protein Name: Histone H4-like protein type G
• Protein Family: Histone H4-like protein
• UniProt Gene Name: HIST1H4G
• UniProt Synonym Gene Names: H4/L; H4FL

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]

Uniprot Description

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling ().