Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Histone H2A type 1-H (HIST1H2AH) Standard Curve.)
Human Histone H2A Type 1-H (HIST1H2AH) ELISA Kit | HIST1H2AH elisa kit
Human Histone H2A Type 1-H (HIST1H2AH) ELISA Kit
Gene Names
HIST1H2AH; H2AH; H2A/S; H2AFALii; dJ86C11.1
Reactivity
Human
Synonyms
Histone H2A Type 1-H (HIST1H2AH); Human Histone H2A Type 1-H (HIST1H2AH) ELISA Kit; HIST1H2AH; H2A/S; H2AFALii; MGC171151; dJ86C11.1; H2A histone family member; HIST1H2AH elisa kit
Reactivity
Human
Specificity
Human Histone H2A type 1-H (HIST1H2AH) ELISA Kit has high sensitivity and excellent specificity for detection of Human HIST1H2AH. No significant cross-reactivity or interference between Human HIST1H2AH and analogues was observed.
Sequence Length
128
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Histone H2A type 1-H (HIST1H2AH) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Histone H2A type 1-H (HIST1H2AH) Standard Curve.)
Related Product Information for HIST1H2AH elisa kit
Description: This Human Histone H2A type 1-H (HIST1H2AH) ELISA Kit employs a two-site sandwich ELISA to quantitate HIST1H2AH in samples. An antibody specific for HIST1H2AH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HIST1H2AH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HIST1H2AH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HIST1H2AH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from HIST1H2AH lack polyA tails but instead contain a palindromic termination element. HIST1H2AH is found in the histone microcluster on chromosome 6p21.33.
Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from HIST1H2AH lack polyA tails but instead contain a palindromic termination element. HIST1H2AH is found in the histone microcluster on chromosome 6p21.33.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
histone H2A type 1-H
NCBI Official Synonym Full Names
histone cluster 1 H2A family member h
NCBI Official Symbol
HIST1H2AH
NCBI Official Synonym Symbols
H2AH; H2A/S; H2AFALii; dJ86C11.1
NCBI Protein Information
histone H2A type 1-H
UniProt Protein Name
Histone H2A type 1-H
Protein Family
UniProt Gene Name
HIST1H2AH
UniProt Synonym Gene Names
HIST1H2AI
UniProt Entry Name
H2A1H_HUMAN
NCBI Description
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq, Aug 2015]
Research Articles on HIST1H2AH
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Product Notes
The Human HIST1H2AH hist1h2ah (Catalog #AAA9715596) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715596 ELISA Kit recognizes Human HIST1H2AH. It is sometimes possible for the material contained within the vial of "Histone H2A Type 1-H (HIST1H2AH), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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