Human Histo-blood group ABO system transferase ELISA Kit | ABO elisa kit

Human Histo-blood group ABO system transferase ELISA Kit

Cat. Num. AAA762759

• Gene Names: ABO; GTB; NAGAT; A3GALNT; A3GALT1
• Reactivity: Human
• Synonyms: Histo-blood group ABO system transferase; Human Histo-blood group ABO system transferase ELISA Kit; ABO/Histo-blood group B transferase (B transferase)/Histo-blood group A transferase (A transferase)/Glycoprotein-fucosylgalactoside alpha-galactosyltransferase)/Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase)/NAGAT/Fucosylglycoprotein 3-alpha-galactosyltransferase/Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase; ABO elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of A2M. No significant cross-reactivity or interference between A2M and analogues was observed.
• Sequence Length: 354

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ug/ml
• Sensitivity: <0.094ug/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level A2M were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level A2M were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ABO elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-A2M antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-A2M antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the A2M amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of A2M can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 58331216
• NCBI GeneID: 28
• NCBI Accession #: NP_065202.2
• NCBI GenBank Nucleotide #: NM_020469.2
• Molecular Weight: 40,934 Da
• NCBI Official Full Name: histo-blood group ABO system transferase
• NCBI Official Synonym Full Names: ABO blood group (transferase A, alpha 1-3-N-acetylgalactosaminyltransferase; transferase B, alpha 1-3-galactosyltransferase)
• NCBI Official Symbol: ABO
• NCBI Official Synonym Symbols: GTB; NAGAT; A3GALNT; A3GALT1
• NCBI Protein Information: histo-blood group ABO system transferase
• UniProt Protein Name: Histo-blood group ABO system transferase
• Protein Family: Histo-blood group ABO system transferase
• UniProt Gene Name: ABO
• UniProt Synonym Gene Names: A transferase; B transferase
• UniProt Entry Name: BGAT_HUMAN

NCBI Description

This gene encodes proteins related to the first discovered blood group system, ABO. Which allele is present in an individual determines the blood group. The 'O' blood group is caused by a deletion of guanine-258 near the N-terminus of the protein which results in a frameshift and translation of an almost entirely different protein. Individuals with the A, B, and AB alleles express glycosyltransferase activities that convert the H antigen into the A or B antigen. Other minor alleles have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ABO: This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity. Belongs to the glycosyltransferase 6 family.

Protein type: Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; Membrane protein, integral; EC 2.4.1.37; Transferase; EC 2.4.1.40

Chromosomal Location of Human Ortholog: 9q34.2

Cellular Component: extracellular region; integral to membrane

Molecular Function: fucosylgalactoside 3-alpha-galactosyltransferase activity; glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase activity; metal ion binding

Biological Process: protein amino acid glycosylation

Disease: Blood Group, Abo System