Human Heterogeneous nuclear ribonucleoproteins C1/C2 (HNRNPC) ELISA Kit | HNRNPC elisa kit

Human Heterogeneous nuclear ribonucleoproteins C1/C2 (HNRNPC) ELISA Kit

Cat. Num. AAA7201822

• Gene Names: HNRNPC; C1; C2; HNRNP; HNRPC; SNRPC
• Reactivity: Human
• Synonyms: Heterogeneous nuclear ribonucleoproteins C1/C2 (HNRNPC); Human Heterogeneous nuclear ribonucleoproteins C1/C2 (HNRNPC) ELISA Kit; HNRNPC elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of HNRNPC. No significant cross-reactivity or interference between HNRNPC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HNRNPC and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 1.0 pg/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HNRNPC elisa kit

Intended Uses: This HNRNPC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human HNRNPC. This ELISA kit for research use only!

Principle of the Assay: HNRNPC ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-HNRNPC antibody and an HNRNPC-HRP conjugate. The assay sample and buffer are incubated together with HNRNPC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HNRNPC concentration since HNRNPC from samples and HNRNPC-HRP conjugate compete for the anti-HNRNPC antibody binding site. Since the number of sites is limited, as more sites are occupied by HNRNPC from the sample, fewer sites are left to bind HNRNPC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HNRNPC concentration in each sample is interpolated from this standard curve.

Product Categories/Family for HNRNPC elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 117189975
• NCBI GeneID: 3183
• NCBI Accession #: NP_112604.2
• NCBI GenBank Nucleotide #: NM_031314.2
• Molecular Weight: 27,822 Da
• NCBI Official Full Name: heterogeneous nuclear ribonucleoproteins C1/C2 isoform a
• NCBI Official Synonym Full Names: heterogeneous nuclear ribonucleoprotein C (C1/C2)
• NCBI Official Symbol: HNRNPC
• NCBI Official Synonym Symbols: C1; C2; HNRNP; HNRPC; SNRPC
• NCBI Protein Information: heterogeneous nuclear ribonucleoproteins C1/C2; hnRNP C1/C2; nuclear ribonucleoprotein particle C1 protein; nuclear ribonucleoprotein particle C2 protein
• UniProt Protein Name: Heterogeneous nuclear ribonucleoproteins C1/C2
• Protein Family: Heterogeneous nuclear ribonucleoprotein
• UniProt Gene Name: HNRNPC
• UniProt Synonym Gene Names: HNRPC; hnRNP C1/C2
• UniProt Entry Name: HNRPC_HUMAN

NCBI Description

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene can act as a tetramer and is involved in the assembly of 40S hnRNP particles. Multiple transcript variants encoding at least two different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

hnRNP C1/C2: a heterogeneous nuclear ribonucleoprotein. May play a role in ribonucleosome assembly by neutralizing basic proteins such as A and B core hnRNP. Contains 1 RNA recognition motif (RRM) domain. Two alternatively spliced isoforms have been described.

Protein type: RNA splicing; Spliceosome; RNA-binding

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: nucleoplasm; spliceosome; protein complex; membrane; nuclear chromatin; nucleus; cytosol

Molecular Function: identical protein binding; protein binding; nucleosomal DNA binding; mRNA 3'-UTR binding; RNA binding; nucleotide binding; poly(U) binding

Biological Process: osteoblast differentiation; nuclear mRNA splicing, via spliceosome; RNA splicing; ATP-dependent chromatin remodeling; gene expression