Human HERV-K_3q12.3 provirus ancestral Gag polyprotein, ERVK5 ELISA Kit | ERVK5 elisa kit

Human HERV-K_3q12.3 provirus ancestral Gag polyprotein, ERVK5 ELISA Kit

Cat. Num. AAA2602647

• Reactivity: Human
• Synonyms: HERV-K_3q12.3 provirus ancestral Gag polyprotein; ERVK5; Human HERV-K_3q12.3 provirus ancestral Gag polyprotein; ERVK5 ELISA Kit; ERVK5 elisa kit

• Reactivity: Human

• Samples: Human serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 50 ng/mL-0.78 ng/mL
• Sensitivity: Up to 0.1 ng/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ERVK5 elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human ERVK5 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 50400656
• UniProt Accession #: Q9HDB9
• Molecular Weight: 73,588 Da
• NCBI Official Full Name: HERV-K_3q12.3 provirus ancestral Gag polyprotein
• UniProt Protein Name: HERV-K_3q12.3 provirus ancestral Gag polyprotein
• UniProt Gene Name: ERVK-5
• UniProt Synonym Gene Names: ERVK5; Gag polyprotein
• UniProt Entry Name: GAK8_HUMAN

Uniprot Description

ERVK5: The products of the Gag polyproteins of infectious retroviruses perform highly complex orchestrated tasks during the assembly, budding, maturation, and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Endogenous Gag proteins may have kept, lost or modified their original function during evolution. Belongs to the beta type-B retroviral Gag protein family. HERV class-II K(HML-2) gag subfamily. 1 isoforms of the human protein are produced by ribosomal frameshifting.

Chromosomal Location of Human Ortholog: 3q12.3

Cellular Component: plasma membrane; viral capsid; nucleus

Molecular Function: nucleic acid binding; zinc ion binding; structural molecule activity

Biological Process: viral reproduction