Human Heparanase (HPA) ELISA Kit | HPA elisa kit

Human Heparanase (HPA) ELISA Kit

Cat. Num. AAA454383

• Gene Names: HPSE; HPA; HPA1; HPR1; HSE1; HPSE1
• Reactivity: Human
• Synonyms: Heparanase (HPA); Human Heparanase (HPA) ELISA Kit; HPSE; HPR1; HPSE1; HSE1; Endo-glucoronidase;; HPA elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of HPA. No significant cross-reactivity or interference between HPA and analogues was observed.
• Sequence Length: 543

• Samples: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 31.25-2000pg/mL
• Sensitivity: 11.3pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HPA were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HPA were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HPA elisa kit

Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of HPA in human tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HPA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to HPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of HPA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 148746204
• NCBI GeneID: 10855
• NCBI Accession #: NP_001092010.1
• NCBI GenBank Nucleotide #: NM_001098540.2
• UniProt Accession #: Q9Y251; Q53GE5; Q9UL39; A9JIG7; C7F7I3; C7F7I4; E9PCA9; E9PGR1
• Molecular Weight: 42,791 Da
• NCBI Official Full Name: heparanase isoform 1 preproprotein
• NCBI Official Synonym Full Names: heparanase
• NCBI Official Symbol: HPSE
• NCBI Official Synonym Symbols: HPA; HPA1; HPR1; HSE1; HPSE1
• NCBI Protein Information: heparanase
• UniProt Protein Name: Heparanase
• UniProt Gene Name: HPSE
• UniProt Synonym Gene Names: HEP; HPA; HPA1; HPR1; HPSE1; HSE1; Hpa1

NCBI Description

Heparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix. The protein encoded by this gene is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. In addition, this cleavage can release bioactive molecules from the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Facilitates cell migration associated with metastasis, wound healing and inflammation. Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas. Acts as procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Increases cell adhesion to the extacellular matrix (ECM), independent of its enzymatic activity. Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion. Heparin increases this AKT1/PKB activation. Regulates osteogenesis. Enhances angiogenesis through up-regulation of SRC-mediated activation of VEGF. Implicated in hair follicle inner root sheath differentiation and hair homeostasis.