Human heparan sulfate (HS) ELISA Kit | HS elisa kit

Human heparan sulfate (HS) ELISA Kit

Cat. Num. AAA267373

• Gene Names: HS3ST1; 3OST; 3OST1
• Reactivity: Human
• Synonyms: heparan sulfate (HS); Human heparan sulfate (HS) ELISA Kit; HS elisa kit

• Reactivity: Human
• Sequence Length: 307

• Samples: Human serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 1000 pg/mL-15.6 pg/mL
• Sensitivity: Up to 5 pg/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for HS elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human HS monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 4826764
• NCBI GeneID: 9957
• NCBI Accession #: NP_005105.1
• NCBI GenBank Nucleotide #: NM_005114.2
• Molecular Weight: 35,773 Da
• NCBI Official Full Name: heparan sulfate glucosamine 3-O-sulfotransferase 1
• NCBI Official Synonym Full Names: heparan sulfate (glucosamine) 3-O-sulfotransferase 1
• NCBI Official Symbol: HS3ST1
• NCBI Official Synonym Symbols: 3OST; 3OST1
• NCBI Protein Information: heparan sulfate glucosamine 3-O-sulfotransferase 1; 3-OST-1; h3-OST-1; heparan sulfate 3-O-sulfotransferase 1; heparin-glucosamine 3-O-sulfotransferase; heparan sulfate D-glucosaminyl 3-O-sulfotransferase 1
• UniProt Protein Name: Heparan sulfate glucosamine 3-O-sulfotransferase 1
• UniProt Gene Name: HS3ST1
• UniProt Synonym Gene Names: 3OST; 3OST1; 3-OST-1; Heparan sulfate 3-O-sulfotransferase 1; h3-OST-1
• UniProt Entry Name: HS3S1_HUMAN

NCBI Description

Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. The enzyme encoded by this gene is a member of the heparan sulfate biosynthetic enzyme family. It possesses both heparan sulfate glucosaminyl 3-O-sulfotransferase activity, anticoagulant heparan sulfate conversion activity, and is a rate limiting enzyme for synthesis of anticoagulant heparan. This enzyme is an intraluminal Golgi resident protein. [provided by RefSeq, Jul 2008]

Uniprot Description

HS3ST1: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to position 3 of glucosamine residues in heparan. Catalyzes the rate limiting step in the biosynthesis of heparan sulfate (HSact). This modification is a crucial step in the biosynthesis of anticoagulant heparan sulfate as it completes the structure of the antithrombin pentasaccharide binding site. Belongs to the sulfotransferase 1 family.

Protein type: Transferase; Glycan Metabolism - heparan sulfate biosynthesis; EC 2.8.2.23

Chromosomal Location of Human Ortholog: 4p16

Cellular Component: Golgi lumen; integral to membrane

Molecular Function: sulfotransferase activity; heparin-glucosamine 3-O-sulfotransferase activity

Biological Process: glycosaminoglycan biosynthetic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; pathogenesis