Human Hemoglobin (HB) ELISA Kit | HB elisa kit

Human Hemoglobin (HB) ELISA Kit

Cat. Num. AAA2700512

• Gene Names: LOC541815; Hb; Hbm; GLB1; GRMZM2G067402
• Reactivity: Human
• Synonyms: Hemoglobin (HB); Human Hemoglobin (HB) ELISA Kit; Hgb; Haemoglobin; Heterotetramer(alphabeta)2; HB elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of hemoglobin. No significant cross-reactivity or interference between hemoglobin and analogues was observed.
• Sequence Length: 165

• Samples: Serum, plasma, tissue homogenates, erythrocyte lysates, cell culture supernates and other biological fluids
• Assay Type: Quantitative Competitive
• Detection Range: 6.17-500ug/mL
• Sensitivity: < 2.62ug/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level hemoglobin were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level hemoglobin were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HB elisa kit

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of hemoglobin in human serum, plasma, tissue homogenates, erythrocyte lysates, cell culture supernates and other biological fluids.

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to hemoglobin has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled hemoglobin and unlabeled hemoglobin (Standards or samples) with the pre-coated antibody specific to hemoglobin. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of hemoglobin in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of hemoglobin in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 162462053
• NCBI GeneID: 541815
• NCBI Accession #: NP_001104966.1
• NCBI GenBank Nucleotide #: NM_001111496.2
• UniProt Accession #: Q9FY42
• Molecular Weight: 18,279 Da
• NCBI Official Full Name: non-symbiotic hemoglobin
• NCBI Official Symbol: LOC541815
• NCBI Official Synonym Symbols: Hb; Hbm; GLB1; GRMZM2G067402
• NCBI Protein Information: non-symbiotic hemoglobin
• UniProt Protein Name: Non-symbiotic hemoglobin
• UniProt Gene Name: HB
• UniProt Synonym Gene Names: GLB1

Uniprot Description

May not function as an oxygen storage or transport protein, but might act as an oxygen sensor or play a role in electron transfer, possibly to a bound oxygen molecule.