Human Helicobacter pylori IgM ELISA Kit
Helicobacter pylori IgM ELISA
Reactivity
Human
Synonyms
Helicobacter pylori IgM; Helicobacter pylori IgM ELISA; Helicobacter pylori IgM elisa kit
Reactivity
Human
Samples
Serum, plasma (EDTA, heparin)
Design
Enzyme immunoassay technique.
Assay Description
1 hour Incubation (RT) + 30 minutes (RT) + 20 minutes at RT (Total clock-time = 1 hour, 50 min.)
Sample Volume
5 uL
Preparation and Storage
2 - 8 degree C
Related Product Information for Helicobacter pylori IgM elisa kit
Description: Enzyme immunoassay for the detection of human IgM antibodies against Helicobacter pylori in serum and plasma
Introduction: Helicobacter pylori, a 2.5-3 um long, twisted or helical gram-negative germ is responsible for 80-90% of Bgastritis cases and is suspected to be a major cofactor for the development of gastric and duodenal ulcers. Up to now, Helicobacter pylori could be most reliably detected by culturing from mucous membrane biopsies; also a urease test was employed for identification. These detection methods are only successful for a relatively high germ count from the inoculate and require an identification directly after taking the biopsy. Recently it was shown that a detection of Helicobacter pylori by indirect immunofluorescence (IIF) should be superior to the above methods. The identification by IIF is also advantageous as it is not necessary to perform the determination directly after the biopsy; thus preparations can be sent to specialized laboratories. The colonisation of the gastric and duodenal mucous membranes by Helicobacter pylori can also be detected serologically by the performance of an enzyme immunoassay (ELISA) or by Western Blot. Patients with confirmed exposition to Helicobacter pylori often show a positive serological result. Since antibodies persist for a linger time after a Helicobacter pylori infection, seropositive individuals are also found among symptomfree patients. The ratio of seropositive values rises with age. By the determination of immunoglobulins in ELISA and by the detection of IgG, IgA and IgM antibodies against specific proteins of Helicobacter pylori in the Western Blot, it should be possible to identify an acute infection with Helicobacter pylori, even if no germs can be found. The detection of Helicobacter pylori is of remarkable interest for the therapy. Bismuth salts and antibiotics use to kill the germs, and these should complement or replace the usual therapy with antacidic and H2 blocking agents. Serological monitoring could thus also be employed to control the success of anti-microbial therapy.
Principle of the Assay: The Helicobacter pylori IgM antibody test kit is based on the principle of the enzyme immunoassay (EIA). Helicobacter antigen is bound on the surface of the microtiter strips. Diluted patient serum or ready-to-use standards are pipetted into the wells of the microtiter plate. A binding between the IgM antibodies of the serum and the immobilized Helicobacter antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use anti-human-IgM peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. The concentration of the IgM antibodies is directly proportional to the intensity of the color. NOTE that samples containing hyper-immune levels of anti-human IgG antibodies may cause false positive results in this assay. Any patient samples testing positive should be pre-diluted with IgG adsorbent and re-tested to confirm elevated levels of IgM.
Intended Use: The Helicobacter pylori IgM Antibody ELISA Test Kit has been designed for the detection of IgM class antibodies against Helicobacter pylori in serum and plasma.
Introduction: Helicobacter pylori, a 2.5-3 um long, twisted or helical gram-negative germ is responsible for 80-90% of Bgastritis cases and is suspected to be a major cofactor for the development of gastric and duodenal ulcers. Up to now, Helicobacter pylori could be most reliably detected by culturing from mucous membrane biopsies; also a urease test was employed for identification. These detection methods are only successful for a relatively high germ count from the inoculate and require an identification directly after taking the biopsy. Recently it was shown that a detection of Helicobacter pylori by indirect immunofluorescence (IIF) should be superior to the above methods. The identification by IIF is also advantageous as it is not necessary to perform the determination directly after the biopsy; thus preparations can be sent to specialized laboratories. The colonisation of the gastric and duodenal mucous membranes by Helicobacter pylori can also be detected serologically by the performance of an enzyme immunoassay (ELISA) or by Western Blot. Patients with confirmed exposition to Helicobacter pylori often show a positive serological result. Since antibodies persist for a linger time after a Helicobacter pylori infection, seropositive individuals are also found among symptomfree patients. The ratio of seropositive values rises with age. By the determination of immunoglobulins in ELISA and by the detection of IgG, IgA and IgM antibodies against specific proteins of Helicobacter pylori in the Western Blot, it should be possible to identify an acute infection with Helicobacter pylori, even if no germs can be found. The detection of Helicobacter pylori is of remarkable interest for the therapy. Bismuth salts and antibiotics use to kill the germs, and these should complement or replace the usual therapy with antacidic and H2 blocking agents. Serological monitoring could thus also be employed to control the success of anti-microbial therapy.
Principle of the Assay: The Helicobacter pylori IgM antibody test kit is based on the principle of the enzyme immunoassay (EIA). Helicobacter antigen is bound on the surface of the microtiter strips. Diluted patient serum or ready-to-use standards are pipetted into the wells of the microtiter plate. A binding between the IgM antibodies of the serum and the immobilized Helicobacter antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use anti-human-IgM peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. The concentration of the IgM antibodies is directly proportional to the intensity of the color. NOTE that samples containing hyper-immune levels of anti-human IgG antibodies may cause false positive results in this assay. Any patient samples testing positive should be pre-diluted with IgG adsorbent and re-tested to confirm elevated levels of IgM.
Intended Use: The Helicobacter pylori IgM Antibody ELISA Test Kit has been designed for the detection of IgM class antibodies against Helicobacter pylori in serum and plasma.
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Product Notes
The Human Helicobacter pylori IgM (Catalog #AAA494240) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA494240 ELISA Kit recognizes Human Helicobacter pylori IgM. It is sometimes possible for the material contained within the vial of "Helicobacter pylori IgM, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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