Human Helicobacter pylori IgM ELISA Kit | IgM elisa kit
Helicobacter pylori IgM ELISA
Reactivity
Human
Synonyms
Helicobacter pylori IgM; Helicobacter pylori IgM ELISA; IgM elisa kit
Reactivity
Human
Specificity
The Bordetella pertussis IgA, IgG, and IgM ELISA was verified in an internal study. The sensitivity and specificity in both cases exceeded 96%.
Application Notes
1 hour (37 degree C) + 30 min. (37 degree C) + 30 min. (37 degree C) = 2 hours total incubation time.
Samples
Various Kinds Of Samples.
Preparation and Storage
Store at 2-8 degree C
Related Product Information for IgM elisa kit
Intended Uses: The Helicobacter pylori IgA, IgG and IgM ELISA tests are qualitative immunoassays for the detection of human antibodies in serum or plasma directed against Helicobacter pylori. These assays are recommended for the sensitive detection of such antibodies in various kinds of samples.
Principle of the Assay: The ELISA (Enzyme Linked Immunosorbent Assay) is an immunoassay, which is particularly suited to the determination of antibodies in the field of infectious serology. The reaction is based on the specific interaction of antibodies with their corresponding antigen. The test strips of the microtiter plate are coated with specific antigens of the pathogen of interest. If antibodies in the sample are present, they bind to the fixed antigen. A secondary antibody, which has been conjugated with the enzyme alkaline phosphatase, detects and binds to the immune complex. The colourless substrate pnitrophenylphosphate is then converted into the coloured product p-nitrophenol. The signal intensity of this reaction product is proportional to the concentration of the analyte in the sample and is measured photometrically!!Background/Introduction: Helicobacter pylori is a spirally formed gram negative bacterium which is highly motile due to it possessed up to seven flagellae. Microbiological identification is based on positive urea, catalase and oxidase tests as well as the absence of hippurate hydrolysis and nitrate reductase. Helicobacter pylori is a very host specific organism. Other Helicobacter species are found in a variety of mammals such as cats, dogs, swine and mice. Phenotypical differences between H. pylori isolates exclusively refer to expression/nonexpression of vacuolating cytotoxin (VacA) and a second toxin which is encoded by cytotoxin-associated gene (CagA). Virulent (Type I) and non-virulent (Type II) Helicobacter strains can be distinguished on the basis of these phenotypical differences.
Principle of the Assay: The ELISA (Enzyme Linked Immunosorbent Assay) is an immunoassay, which is particularly suited to the determination of antibodies in the field of infectious serology. The reaction is based on the specific interaction of antibodies with their corresponding antigen. The test strips of the microtiter plate are coated with specific antigens of the pathogen of interest. If antibodies in the sample are present, they bind to the fixed antigen. A secondary antibody, which has been conjugated with the enzyme alkaline phosphatase, detects and binds to the immune complex. The colourless substrate pnitrophenylphosphate is then converted into the coloured product p-nitrophenol. The signal intensity of this reaction product is proportional to the concentration of the analyte in the sample and is measured photometrically!!Background/Introduction: Helicobacter pylori is a spirally formed gram negative bacterium which is highly motile due to it possessed up to seven flagellae. Microbiological identification is based on positive urea, catalase and oxidase tests as well as the absence of hippurate hydrolysis and nitrate reductase. Helicobacter pylori is a very host specific organism. Other Helicobacter species are found in a variety of mammals such as cats, dogs, swine and mice. Phenotypical differences between H. pylori isolates exclusively refer to expression/nonexpression of vacuolating cytotoxin (VacA) and a second toxin which is encoded by cytotoxin-associated gene (CagA). Virulent (Type I) and non-virulent (Type II) Helicobacter strains can be distinguished on the basis of these phenotypical differences.
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Product Notes
The Human IgM (Catalog #AAA490277) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA490277 ELISA Kit recognizes Human IgM. 1 hour (37 degree C) + 30 min. (37 degree C) + 30 min. (37 degree C) = 2 hours total incubation time. Researchers should empirically determine the suitability of the IgM for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Helicobacter pylori IgM, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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