Human Growth Hormone 2 (GH2) ELISA Kit | GH2 elisa kit

Human Growth Hormone 2 (GH2) ELISA Kit

Cat. Num. AAA2704795

• Gene Names: GH2; GHL; GHV; GH-V; GHB2; hGH-V
• Reactivity: Human
• Synonyms: Growth Hormone 2 (GH2); Human Growth Hormone 2 (GH2) ELISA Kit; GH-V; GHL; GHV; hGH-V; Growth Hormone Variant; Placenta-Specific Growth Hormone; Placental Specific Growth Hormone; GH2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Growth Hormone 2 (GH2).; No significant cross-reactivity or interference between Growth Hormone 2 (GH2) and analogues was observed.
• Sequence Length: 245

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 78-5000pg/mL
• Sensitivity: < 32pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Hormone 2 (GH2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Hormone 2 (GH2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GH2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Hormone 2 (GH2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Hormone 2 (GH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Hormone 2 (GH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Growth Hormone 2 (GH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 18088461
• NCBI GeneID: 2689
• UniProt Accession #: P01242; O14643; O14644; P09587; B1A4H5; B1A4H7
• Molecular Weight: 27,101 Da
• NCBI Official Full Name: Growth hormone 2
• NCBI Official Synonym Full Names: growth hormone 2
• NCBI Official Symbol: GH2
• NCBI Official Synonym Symbols: GHL; GHV; GH-V; GHB2; hGH-V
• NCBI Protein Information: growth hormone variant
• UniProt Protein Name: Growth hormone variant
• Protein Family: Growth hormone
• UniProt Gene Name: GH2
• UniProt Synonym Gene Names: GH-V

NCBI Description

The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. As in the case of its pituitary counterpart, growth hormone 1, the predominant isoform of this particular family member shows similar somatogenic activity, with reduced lactogenic activity. Mutations in this gene lead to placental growth hormone/lactogen deficiency. [provided by RefSeq, Jul 2008]

Uniprot Description

Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.