Human GRIN2B ELISA Kit | GRIN2B elisa kit

Human GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2B) ELISA Kit

Cat. Num. AAA2516105

• Gene Names: GRIN2B; MRD6; NR2B; hNR3; EIEE27; GluN2B; NMDAR2B
• Reactivity: Human
• Synonyms: GRIN2B; Human GRIN2B (Glutamate Receptor; Ionotropic; N-Methyl-D-Aspartate 2B) ELISA Kit; GRIN2B elisa kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant Human GRIN2B. No significant cross-reactivity or interference between Human GRIN2B and analogues was observed.
• Sequence Length: 1484

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.313-20ng/mL
• Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL
• Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human GRIN2B concentrations in serum, plasma and other biological fluids.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GRIN2B elisa kit

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GRIN2B. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GRIN2B and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GRIN2B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of GRIN2B. You can calculate the concentration of GRIN2B in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 167003331
• NCBI GeneID: 2904
• NCBI Accession #: NP_000825.2
• NCBI GenBank Nucleotide #: NM_000834.3
• Molecular Weight: 166,367 Da
• NCBI Official Full Name: glutamate receptor ionotropic, NMDA 2B
• NCBI Official Synonym Full Names: glutamate receptor, ionotropic, N-methyl D-aspartate 2B
• NCBI Official Symbol: GRIN2B
• NCBI Official Synonym Symbols: MRD6; NR2B; hNR3; EIEE27; GluN2B; NMDAR2B
• NCBI Protein Information: glutamate receptor ionotropic, NMDA 2B; N-methyl D-aspartate receptor subtype 2B; N-methyl-D-aspartate receptor subunit 3; NR3; glutamate [NMDA] receptor subunit epsilon-2; glutamate receptor subunit epsilon-2
• UniProt Protein Name: Glutamate receptor ionotropic, NMDA 2B
• Protein Family: Glutamate receptor ionotropic
• UniProt Gene Name: GRIN2B
• UniProt Synonym Gene Names: NMDAR2B; GluN2B; NMDAR2B; NR2B; NR3; hNR3
• UniProt Entry Name: NMDE2_HUMAN

NCBI Description

N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of three different subunits: NR1 (GRIN1), NR2 (GRIN2A, GRIN2B, GRIN2C, or GRIN2D) and NR3 (GRIN3A or GRIN3B). The NR2 subunit acts as the agonist binding site for glutamate. This receptor is the predominant excitatory neurotransmitter receptor in the mammalian brain. [provided by RefSeq, Jul 2008]

Uniprot Description

NMDAR2B: an NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. Mediates neuronal functions in glutamate neurotransmission. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death.

Protein type: Membrane protein, integral; Channel, ligand-gated; Channel, calcium; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 12p12

Cellular Component: postsynaptic membrane; synaptic vesicle; neuron projection; cell surface; integral to plasma membrane; dendrite; plasma membrane; cell junction; N-methyl-D-aspartate selective glutamate receptor complex

Molecular Function: protein binding; extracellular-glutamate-gated ion channel activity; zinc ion binding; N-methyl-D-aspartate selective glutamate receptor activity; calcium channel activity; glycine binding

Biological Process: axon guidance; synaptic transmission, glutamatergic; startle response; behavioral fear response; in utero embryonic development; glutamate signaling pathway; regulation of synaptic plasticity; learning; memory; synaptic transmission; detection of mechanical stimulus involved in sensory perception of pain; behavioral response to pain; response to ethanol; learning and/or memory; sensory organ development; suckling behavior; transport; ionotropic glutamate receptor signaling pathway; ephrin receptor signaling pathway; regulation of excitatory postsynaptic membrane potential

Disease: Mental Retardation, Autosomal Dominant 6; Epileptic Encephalopathy, Early Infantile, 27