Human GRIA1 (Glutamate receptor 1) ELISA Kit | GRIA1 elisa kit

Human GRIA1 (Glutamate receptor 1) ELISA Kit

Cat. Num. AAA7606063

• Gene Names: Gria1; Glr1; Glr-1; GluA1; GluRA; Glur1; HIPA1; GluR-A; Glur-1; gluR-K1; 2900051M01Rik
• Reactivity: Human
• Synonyms: GRIA1 (Glutamate receptor 1); Human GRIA1 (Glutamate receptor 1) ELISA Kit; Glutamate receptor 1; GRIA1 elisa kit

• Reactivity: Human
• Sequence Length: 907

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GRIA1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- GRIA1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-GRIA1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the GRIA1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of GRIA1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 164664512
• NCBI GeneID: 14799
• NCBI Accession #: NP_001106796.1
• NCBI GenBank Nucleotide #: NM_001113325.2
• UniProt Accession #: P23818
• Molecular Weight: 101,569 Da
• NCBI Official Full Name: glutamate receptor 1 isoform 1
• NCBI Official Synonym Full Names: glutamate receptor, ionotropic, AMPA1 (alpha 1)
• NCBI Official Symbol: Gria1
• NCBI Official Synonym Symbols: Glr1; Glr-1; GluA1; GluRA; Glur1; HIPA1; GluR-A; Glur-1; gluR-K1; 2900051M01Rik
• NCBI Protein Information: glutamate receptor 1
• UniProt Protein Name: Glutamate receptor 1
• UniProt Gene Name: Gria1
• UniProt Synonym Gene Names: Glur1; GluR-1; GluA1

Uniprot Description

GluR1: an integral membrane protein belonging to the glutamate-gated ion channel family. L-glutamate (Glu) acts as an excitatory neurotransmitter at many synapses in the central nervous system. Glutamate receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. Each of the four GluR proteins (GRIA1-4) include flip and flop isoforms generated by alternative RNA splicing. Expressed in granule and pyramidal cells in the hippocampal formation.

Protein type: Channel, calcium; Channel, ligand-gated; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 11 B1.3|11 34.51 cM

Cellular Component: asymmetric synapse; cell soma; cell surface; cytosol; dendrite; dendritic shaft; dendritic spine; early endosome; endoplasmic reticulum; excitatory synapse; ionotropic glutamate receptor complex; membrane; neuromuscular junction; neuron projection; plasma membrane; postsynaptic density; postsynaptic membrane; protein complex; recycling endosome; synapse; synaptic vesicle

Molecular Function: adenylate cyclase binding; alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity; beta-2 adrenergic receptor binding; G-protein alpha-subunit binding; G-protein beta-subunit binding; identical protein binding; ionotropic glutamate receptor activity; myosin V binding; PDZ domain binding; protein binding; protein domain specific binding; protein kinase A binding; protein kinase binding; small GTPase binding

Biological Process: generation of action potential; long-term memory; positive regulation of membrane potential; positive regulation of synaptic transmission; receptor internalization; regulation of postsynaptic membrane potential; regulation of receptor recycling; regulation of synaptic plasticity; regulation of synaptic transmission; response to toxin; synaptic transmission