Human Gonadotropin Releasing Hormone (GnRH) ELISA Kit | GnRH elisa kit

Human Gonadotropin Releasing Hormone (GnRH) ELISA Kit

Cat. Num. AAA454389

• Gene Names: GNRH1; GRH; GNRH; LHRH; LNRH
• Reactivity: Human
• Synonyms: Gonadotropin Releasing Hormone (GnRH); Human Gonadotropin Releasing Hormone (GnRH) ELISA Kit; GNRH1; GRH; LNRH; LHRH; LH-RH I; Luliberin; Luteinizing-Hormone Releasing Hormone; Progonadoliberin-1; Gonadoliberin I; Gonadorelin; GnRH-associated peptide 1; GnRH elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of GNRH. No significant cross-reactivity or interference between GNRH and analogues was observed.
• Sequence Length: 92

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Competitive inhibition
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.064ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GNRH were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GNRH were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Related Product Information for GnRH elisa kit

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of GNRH in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to GNRH has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled GNRH and unlabeled GNRH (Standards or samples) with the pre-coated antibody specific to GNRH. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of GNRH in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of GNRH in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 133908612
• NCBI GeneID: 2796
• NCBI Accession #: NP_001076580.1
• NCBI GenBank Nucleotide #: NM_001083111.1
• UniProt Accession #: P01148; A0AVP0
• Molecular Weight: 10,380 Da
• NCBI Official Full Name: progonadoliberin-1 isoform 2 preproprotein
• NCBI Official Synonym Full Names: gonadotropin releasing hormone 1
• NCBI Official Symbol: GNRH1
• NCBI Official Synonym Symbols: GRH; GNRH; LHRH; LNRH
• NCBI Protein Information: progonadoliberin-1
• UniProt Protein Name: Progonadoliberin-1
• UniProt Gene Name: GNRH1
• UniProt Synonym Gene Names: GNRH; GRH; LHRH; GnRH-I; LH-RH I

NCBI Description

This gene encodes a preproprotein that is proteolytically processed to generate a peptide that is a member of the gonadotropin-releasing hormone (GnRH) family of peptides. Alternative splicing results in multiple transcript variants, at least one of which is secreted and then cleaved to generate gonadoliberin-1 and GnRH-associated peptide 1. Gonadoliberin-1 stimulates the release of luteinizing and follicle stimulating hormones, which are important for reproduction. Mutations in this gene are associated with hypogonadotropic hypogonadism. [provided by RefSeq, Nov 2015]

Uniprot Description

Stimulates the secretion of gonadotropins; it stimulates the secretion of both luteinizing and follicle-stimulating hormones.