Human Glycophorin A (GYPA) ELISA Kit | GYPA elisa kit

Human Glycophorin A (GYPA) ELISA Kit

Cat. Num. AAA2023242

• Gene Names: GYPA; MN; GPA; MNS; GPSAT; PAS-2; CD235a; GPErik; HGpMiV; HGpMiXI; HGpSta(C)
• Reactivity: Human
• Synonyms: Glycophorin A (GYPA); Human Glycophorin A (GYPA) ELISA Kit; CD235a; MN; MNS; GPA; GPErik; GPSAT; PAS-2; GpMiIII; HGpMiIII; HGpMiV; HGpMiX; HGpMiXI; HGpSta; Sialoglycoprotein alpha; MN sialoglycoprotein; GYPA elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Glycophorin A (GYPA).; No significant cross-reactivity or interference between Glycophorin A (GYPA) and analogues was observed.
• Sequence Length: 117

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 15.6-1000pg/mL
• Sensitivity: < 5.5pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glycophorin A (GYPA) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glycophorin A (GYPA) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GYPA elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycophorin A (GYPA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycophorin A (GYPA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycophorin A (GYPA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glycophorin A (GYPA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for GYPA elisa kit

Infection immunity; Hematology; Developmental science

NCBI and Uniprot Product Information

• NCBI GI #: 298286506
• NCBI GeneID: 2993
• NCBI Accession #: NP_002090.4
• NCBI GenBank Nucleotide #: NM_002099.7
• UniProt Accession #: P02724; Q9BS51; A8K3E6; B8Q182; B8Q185
• Molecular Weight: 13,000 Da
• NCBI Official Full Name: glycophorin-A isoform 1
• NCBI Official Synonym Full Names: glycophorin A (MNS blood group)
• NCBI Official Symbol: GYPA
• NCBI Official Synonym Symbols: MN; GPA; MNS; GPSAT; PAS-2; CD235a; GPErik; HGpMiV; HGpMiXI; HGpSta(C)
• NCBI Protein Information: glycophorin-A
• UniProt Protein Name: Glycophorin-A
• Protein Family: Glycophorin
• UniProt Gene Name: GYPA
• UniProt Synonym Gene Names: GPA
• UniProt Entry Name: GLPA_HUMAN

NCBI Description

Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. In addition to the M or N and S or s antigens that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta, as well as Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. [provided by RefSeq, Jul 2008]

Uniprot Description

GYPA: Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV). Belongs to the glycophorin A family.

Protein type: Adaptor/scaffold; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q31.21

Cellular Component: membrane; integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: viral receptor activity; identical protein binding; protein homodimerization activity

Biological Process: entry of virus into host cell; cytoskeletal anchoring; regulation of response to osmotic stress

Disease: Malaria, Susceptibility To