Human Glutathione Synthetase (GSS) ELISA Kit | GSS elisa kit

Human Glutathione Synthetase (GSS) ELISA Kit

Cat. Num. AAA2023523

• Gene Names: GSS; GSHS; HEL-S-64p; HEL-S-88n
• Reactivity: Human
• Synonyms: Glutathione Synthetase (GSS); Human Glutathione Synthetase (GSS) ELISA Kit; Glutathione synthase; GSS elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Glutathione Synthetase (GSS).; No significant cross-reactivity or interference between Glutathione Synthetase (GSS) and analogues was observed.
• Sequence Length: 363

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.115ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutathione Synthetase (GSS) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutathione Synthetase (GSS) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GSS elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione Synthetase (GSS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutathione Synthetase (GSS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione Synthetase (GSS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutathione Synthetase (GSS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for GSS elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 4504169
• NCBI GeneID: 2937
• NCBI Accession #: NP_000169.1
• NCBI GenBank Nucleotide #: NM_000178.2
• UniProt Accession #: P48637; Q4TTD9; B2R697; B6F210; E1P5P9
• Molecular Weight: 40,349 Da
• NCBI Official Full Name: glutathione synthetase
• NCBI Official Synonym Full Names: glutathione synthetase
• NCBI Official Symbol: GSS
• NCBI Official Synonym Symbols: GSHS; HEL-S-64p; HEL-S-88n
• NCBI Protein Information: glutathione synthetase
• UniProt Protein Name: Glutathione synthetase
• Protein Family: Glutathione synthetase
• UniProt Gene Name: GSS
• UniProt Synonym Gene Names: GSH synthetase; GSH-S
• UniProt Entry Name: GSHB_HUMAN

NCBI Description

Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency. [provided by RefSeq, Jul 2008]

Uniprot Description

GSS: Defects in GSS are the cause of glutathione synthetase deficiency (GSS deficiency); also known as 5- oxoprolinuria or pyroglutamic aciduria. It is a severe form characterized by an increased rate of hemolysis and defective function of the central nervous system. Defects in GSS are the cause of glutathione synthetase deficiency of erythrocytes (GLUSYNDE)[MIM:231900]. Glutathione synthetase deficiency of erythrocytes is a mild form causing hemolytic anemia. Belongs to the eukaryotic GSH synthase family.

Protein type: Other Amino Acids Metabolism - glutathione; Ligase; EC 6.3.2.3

Chromosomal Location of Human Ortholog: 20q11.2

Cellular Component: cytosol

Molecular Function: protein homodimerization activity; glutathione binding; glycine binding; magnesium ion binding; glutathione synthase activity; ATP binding

Biological Process: response to nutrient levels; amino acid metabolic process; nervous system development; response to cadmium ion; xenobiotic metabolic process; glutathione biosynthetic process; response to oxidative stress; response to amino acid stimulus; aging

Disease: Glutathione Synthetase Deficiency; Glutathione Synthetase Deficiency Of Erythrocytes, Hemolytic Anemia Due To