Human Glutathione S Transferase Pi (GSTp) ELISA Kit | GSTp elisa kit

Human Glutathione S Transferase Pi (GSTp) ELISA Kit

Cat. Num. AAA2022711

• Gene Names: GSTP1; PI; DFN7; GST3; GSTP; FAEES3; HEL-S-22
• Reactivity: Human
• Synonyms: Glutathione S Transferase Pi (GSTp); Human Glutathione S Transferase Pi (GSTp) ELISA Kit; GSTP1; GST-P1; DFN7; FAEES3; GST3; GSTp elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Glutathione S Transferase Pi (GSTp).; No significant cross-reactivity or interference between Glutathione S Transferase Pi (GSTp) and analogues was observed.
• Sequence Length: 210

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.056ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutathione S Transferase Pi (GSTp) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutathione S Transferase Pi (GSTp) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GSTp elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione S Transferase Pi (GSTp). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutathione S Transferase Pi (GSTp). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione S Transferase Pi (GSTp), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutathione S Transferase Pi (GSTp) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for GSTp elisa kit

Enzyme & Kinase; Metabolic pathway; Tumor immunity; Hepatology

NCBI and Uniprot Product Information

• NCBI GI #: 32187525
• NCBI GeneID: 2950
• UniProt Accession #: P09211; O00460; Q15690; Q5TZY3
• Molecular Weight: 23,356 Da
• NCBI Official Full Name: glutathione S-transferase pi
• NCBI Official Synonym Full Names: glutathione S-transferase pi 1
• NCBI Official Symbol: GSTP1
• NCBI Official Synonym Symbols: PI; DFN7; GST3; GSTP; FAEES3; HEL-S-22
• NCBI Protein Information: glutathione S-transferase P
• UniProt Protein Name: Glutathione S-transferase P
• UniProt Gene Name: GSTP1
• UniProt Synonym Gene Names: FAEES3; GST3
• UniProt Entry Name: GSTP1_HUMAN

NCBI Description

Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. [provided by RefSeq, Jul 2008]

Uniprot Description

GSTP1: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration. Homodimer. Interacts with CDK5. Belongs to the GST superfamily. Pi family.

Protein type: Xenobiotic Metabolism - metabolism by cytochrome P450; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Other Amino Acids Metabolism - glutathione; EC 2.5.1.18; Transferase

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: extracellular space; mitochondrion; cytoplasm; plasma membrane; intracellular; nucleus; cytosol; vesicle

Molecular Function: JUN kinase binding; protein binding; glutathione transferase activity; kinase regulator activity

Biological Process: negative regulation of JNK activity; negative regulation of MAP kinase activity; central nervous system development; negative regulation of MAPKKK cascade; negative regulation of acute inflammatory response; negative regulation of interleukin-1 beta production; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of tumor necrosis factor production; negative regulation of stress-activated MAPK cascade; regulation of stress-activated MAPK cascade; response to reactive oxygen species; negative regulation of biosynthetic process; glutathione metabolic process; negative regulation of fibroblast proliferation; negative regulation of nitric-oxide synthase biosynthetic process; xenobiotic metabolic process; positive regulation of superoxide release; negative regulation of protein kinase activity; negative regulation of apoptosis