Human Glutathione S Transferase Kappa 1 (GSTk1) ELISA Kit | GSTk1 elisa kit
Human Glutathione S Transferase Kappa 1 (GSTk1) ELISA Kit
Gene Names
Gstk1; DsbA-L; AW260476; 0610025I19Rik
Reactivity
Human
Synonyms
Glutathione S Transferase Kappa 1 (GSTk1); Human Glutathione S Transferase Kappa 1 (GSTk1) ELISA Kit; ELISA Kit for Glutathione S Transferase Kappa 1 (GSTk1); GSTk1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Glutathione S Transferase Kappa 1 (GSTk1).
No significant cross-reactivity or interference between Glutathione S Transferase Kappa 1 (GSTk1) and analogues was observed.
No significant cross-reactivity or interference between Glutathione S Transferase Kappa 1 (GSTk1) and analogues was observed.
Samples
Serum, plasma and other biological fluids.
Assay Type
Sandwich
Detection Range
1.563-100 U/L The standard curve concentrations used for the ELISA's were 100 U/L, 50 U/L, 25 U/L, 12.5 U/L, 6.25 U/L, 3.125 U/L, 1.563 U/L
Sensitivity
Typically less than 1 U/L.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutathione S Transferase Kappa 1 (GSTk1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutathione S Transferase Kappa 1 (GSTk1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutathione S Transferase Kappa 1 (GSTk1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all the reagents should be kept according to the label on each vial. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C while others should be at 4 degree C.
Related Product Information for GSTk1 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione S Transferase Kappa 1 (GSTk1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutathione S Transferase Kappa 1 (GSTk1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione S Transferase Kappa 1 (GSTk1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutathione S Transferase Kappa 1 (GSTk1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
glutathione S-transferase kappa 1
NCBI Official Synonym Full Names
glutathione S-transferase kappa 1
NCBI Official Symbol
Gstk1
NCBI Official Synonym Symbols
DsbA-L; AW260476; 0610025I19Rik
NCBI Protein Information
glutathione S-transferase kappa 1
Protein Family
Research Articles on GSTk1
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Product Notes
The Human GSTk1 (Catalog #AAA2000177) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2000177 ELISA Kit recognizes Human GSTk1. It is sometimes possible for the material contained within the vial of "Glutathione S Transferase Kappa 1 (GSTk1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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