Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Glutamyl-tRNA (QRSL1) Standard Curve.)
Human Glutamyl-tRNA (QRSL1) ELISA Kit | QRSL1 elisa kit
Human Glutamyl-tRNA (QRSL1) ELISA Kit
Gene Names
QRSL1; GatA
Reactivity
Human
Synonyms
Glutamyl-tRNA (QRSL1); Human Glutamyl-tRNA (QRSL1) ELISA Kit; QRSL1; DKFZp564C1278; FLJ10989; FLJ12189; FLJ13447; GatA; QRSL1 elisa kit
Reactivity
Human
Specificity
Human Glutamyl-tRNA (QRSL1) ELISA Kit has high sensitivity and excellent specificity for detection of Human QRSL1. No significant cross-reactivity or interference between Human QRSL1 and analogues was observed.
Sequence Length
528
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Glutamyl-tRNA (QRSL1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Glutamyl-tRNA (QRSL1) Standard Curve.)
Related Product Information for QRSL1 elisa kit
Description: This Human Glutamyl-tRNA (QRSL1) ELISA Kit employs a two-site sandwich ELISA to quantitate QRSL1 in samples. An antibody specific for QRSL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any QRSL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for QRSL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of QRSL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: QRSL1 belongs to the amidase family, similar to glutaminyl-tRNA synthetase. Glutaminyl-tRNA synthetase is a class Ic synthetase and shows several similarities with glutamyl-tRNA synthetase concerning structure and catalytic properties. It is an alpha2 dimer. Glutaminyl-tRNA synthetase is a relatively rare synthetase, found in the cytosolic compartment of eukaryotes, in Escherichia coli and a number of other Gram-negative bacteria, and in Deinococcus radiodurans. In contrast, the pathway to Gln-tRNA in mitochondria, Archaea, Gram-positive bacteria, and a number of other lineages is by misacylation with Glu followed by transamidation to correct the aminoacylation to Gln. A stable glutaminly-adenylate analog, which inhibits GlnRS with a Ki of 1.32 microM, was synthesized and cocrystallized with GlnRS and tRNA2Gln.
Background: QRSL1 belongs to the amidase family, similar to glutaminyl-tRNA synthetase. Glutaminyl-tRNA synthetase is a class Ic synthetase and shows several similarities with glutamyl-tRNA synthetase concerning structure and catalytic properties. It is an alpha2 dimer. Glutaminyl-tRNA synthetase is a relatively rare synthetase, found in the cytosolic compartment of eukaryotes, in Escherichia coli and a number of other Gram-negative bacteria, and in Deinococcus radiodurans. In contrast, the pathway to Gln-tRNA in mitochondria, Archaea, Gram-positive bacteria, and a number of other lineages is by misacylation with Glu followed by transamidation to correct the aminoacylation to Gln. A stable glutaminly-adenylate analog, which inhibits GlnRS with a Ki of 1.32 microM, was synthesized and cocrystallized with GlnRS and tRNA2Gln.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial
NCBI Official Synonym Full Names
glutaminyl-tRNA amidotransferase subunit QRSL1
NCBI Official Symbol
QRSL1
NCBI Official Synonym Symbols
GatA
NCBI Protein Information
glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial
UniProt Protein Name
Glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial
UniProt Gene Name
QRSL1
UniProt Synonym Gene Names
Glu-AdT subunit A
UniProt Entry Name
GATA_HUMAN
Research Articles on QRSL1
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Product Notes
The Human QRSL1 qrsl1 (Catalog #AAA9714663) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714663 ELISA Kit recognizes Human QRSL1. It is sometimes possible for the material contained within the vial of "Glutamyl-tRNA (QRSL1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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