Human Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1) ELISA Kit | GRIN1 elisa kit

Human Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1) ELISA Kit

Cat. Num. AAA2705631

• Gene Names: GRIN1; NR1; MRD8; GluN1; NMDA1; NMD-R1; NMDAR1
• Reactivity: Human
• Synonyms: Glutamate Receptor; Ionotropic; N-Methyl-D-Aspartate 1 (GRIN1); Human Glutamate Receptor; N-Methyl-D-Aspartate 1 (GRIN1) ELISA Kit; NMDA1; NMDAR1; NR1; Glutamate [NMDA] receptor subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1; GRIN1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1).; No significant cross-reactivity or interference between Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1) and analogues was observed.
• Sequence Length: 938

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.115ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GRIN1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 11038637
• NCBI GeneID: 2902
• NCBI Accession #: NP_015566.1
• NCBI GenBank Nucleotide #: NM_007327.3
• UniProt Accession #: Q05586; P35437; Q12867; Q12868; Q5VSF3; Q5VSF4; Q5VSF5; Q5VSF6; Q5VSF7; A6NLK7; A6NLR1; C9K0X1
• Molecular Weight: 101,851 Da
• NCBI Official Full Name: glutamate receptor ionotropic, NMDA 1 isoform GluN1-1a
• NCBI Official Synonym Full Names: glutamate ionotropic receptor NMDA type subunit 1
• NCBI Official Symbol: GRIN1
• NCBI Official Synonym Symbols: NR1; MRD8; GluN1; NMDA1; NMD-R1; NMDAR1
• NCBI Protein Information: glutamate receptor ionotropic, NMDA 1
• UniProt Protein Name: Glutamate receptor ionotropic, NMDA 1
• Protein Family: Glutamate receptor ionotropic
• UniProt Gene Name: GRIN1
• UniProt Synonym Gene Names: NMDAR1; GluN1; NMD-R1

NCBI Description

The protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

NMDAR1: a subunit of N-methyl-D-aspartate (NMDA) receptors, members of the glutamate receptor channel superfamily. Possesses high calcium permeability and voltage-dependent sensitivity to magnesium and is modulated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. Mediates neuronal functions in glutamate neurotransmission. Three alternatively-spliced isoforms have been described.

Protein type: Channel, calcium; Channel, ligand-gated; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: cell junction; cell surface; dendrite; dendritic spine; endoplasmic reticulum; excitatory synapse; integral component of plasma membrane; neuron projection; NMDA selective glutamate receptor complex; plasma membrane; postsynaptic density; postsynaptic density membrane; postsynaptic membrane; synapse; synaptic vesicle; terminal bouton

Molecular Function: calcium channel activity; calcium ion binding; calmodulin binding; extracellular-glutamate-gated ion channel activity; glutamate binding; glutamate-gated calcium ion channel activity; glycine binding; neurotransmitter binding; NMDA glutamate receptor activity; protein binding; protein complex binding; Ras guanyl-nucleotide exchange factor activity; receptor binding; transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential

Biological Process: adult locomotory behavior; calcium ion homeostasis; calcium-mediated signaling; cation transport; cellular response to amyloid-beta; cerebral cortex development; conditioned taste aversion; ephrin receptor signaling pathway; excitatory postsynaptic potential; ionotropic glutamate receptor signaling pathway; long-term memory; male mating behavior; MAPK cascade; negative regulation of neuron apoptosis; olfactory learning; pons maturation; positive regulation of apoptosis; positive regulation of calcium ion transport into cytosol; positive regulation of excitatory postsynaptic potential; positive regulation of transcription from RNA polymerase II promoter; prepulse inhibition; propylene metabolic process; protein heterotetramerization; regulation of axonogenesis; regulation of dendrite morphogenesis; regulation of long-term neuronal synaptic plasticity; regulation of membrane potential; regulation of molecular function; regulation of respiratory gaseous exchange; regulation of synapse assembly; respiratory gaseous exchange; response to amphetamine; response to ethanol; response to glycine; response to morphine; sensory perception of pain; social behavior; suckling behavior; synaptic transmission, glutamatergic; visual learning

Disease: Mental Retardation, Autosomal Dominant 8