Human Glutamate Receptor Interacting Protein 1 (GRIP1) ELISA Kit | GRIP1 elisa kit

Human Glutamate Receptor Interacting Protein 1 (GRIP1) ELISA Kit

Cat. Num. AAA2703664

• Gene Names: GRIP1; GRIP; FRASRS3
• Reactivity: Human
• Synonyms: Glutamate Receptor Interacting Protein 1 (GRIP1); Human Glutamate Receptor Interacting Protein 1 (GRIP1) ELISA Kit; GRIP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Glutamate Receptor Interacting Protein 1 (GRIP1).; No significant cross-reactivity or interference between Glutamate Receptor Interacting Protein 1 (GRIP1) and analogues was observed.
• Sequence Length: 1,076

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.125ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutamate Receptor Interacting Protein 1 (GRIP1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutamate Receptor Interacting Protein 1 (GRIP1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GRIP1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate Receptor Interacting Protein 1 (GRIP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutamate Receptor Interacting Protein 1 (GRIP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutamate Receptor Interacting Protein 1 (GRIP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutamate Receptor Interacting Protein 1 (GRIP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 115527660
• NCBI GeneID: 23426
• UniProt Accession #: Q9Y3R0; Q1RLM0; C9JT59
• Molecular Weight: 116,371 Da
• NCBI Official Full Name: Glutamate receptor interacting protein 1
• NCBI Official Synonym Full Names: glutamate receptor interacting protein 1
• NCBI Official Symbol: GRIP1
• NCBI Official Synonym Symbols: GRIP; FRASRS3
• NCBI Protein Information: glutamate receptor-interacting protein 1
• UniProt Protein Name: Glutamate receptor-interacting protein 1
• Protein Family: Glutamate receptor-interacting protein
• UniProt Gene Name: GRIP1
• UniProt Synonym Gene Names: GRIP-1

NCBI Description

This gene encodes a member of the glutamate receptor interacting protein family. The encoded scaffold protein binds to and mediates the trafficking and membrane organization of a number of transmembrane proteins. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, May 2010]

Uniprot Description

May play a role as a localized scaffold for the assembly of a multiprotein signaling complex and as mediator of the trafficking of its binding partners at specific subcellular location in neurons (PubMed:10197531). Through complex formation with NSG1, GRIA2 and STX12 controls the intracellular fate of AMPAR and the endosomal sorting of the GRIA2 subunit toward recycling and membrane targeting ().