Human Gelsolin (GSN) ELISA Kit | GSN elisa kit

Human Gelsolin (GSN) Wide-range ELISA Kit

Cat. Num. AAA2709265

• Gene Names: GSN; ADF; AGEL
• Reactivity: Human
• Applications: ELISA
• Synonyms: Gelsolin (GSN); Human Gelsolin (GSN) Wide-range ELISA Kit; Gelsolin; GS; AGEL; ADF; Brevin; Gelsolin Amyloidosis; Finnish Type; Actin-depolymerizing factor; GSN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Wide-range Gelsolin (GSN).; No significant cross-reactivity or interference between Wide-range Gelsolin (GSN) and analogues was observed.
• Sequence Length: 782

• Applicable Applications for GSN elisa kit: ELISA (EIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 6.25-400ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 2.83ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Gelsolin (GSN) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Gelsolin (GSN) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wide-range Gelsolin (GSN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wide-range Gelsolin (GSN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wide-range Gelsolin (GSN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Wide-range Gelsolin (GSN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 121116
• NCBI GeneID: 2934
• UniProt Accession #: P06396
• NCBI Official Full Name: Gelsolin
• NCBI Official Synonym Full Names: gelsolin
• NCBI Official Symbol: GSN
• NCBI Official Synonym Symbols: ADF; AGEL
• NCBI Protein Information: gelsolin
• UniProt Protein Name: Gelsolin
• UniProt Gene Name: GSN
• UniProt Synonym Gene Names: ADF
• UniProt Entry Name: GELS_HUMAN

NCBI Description

The protein encoded by this gene binds to the "plus" ends of actin monomers and filaments to prevent monomer exchange. The encoded calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this gene are a cause of familial amyloidosis Finnish type (FAF). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Gelsolin: a calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Its expression is significantly decreased in many tumors and transformed cell lines. Gelsolin and other proteins including E-cadherin, BRCA, p21WAF, HoxA5, and retinoic acid receptor II are regulated by epigenetic changes in breast cancers. Gelsolin helps mediate the ability of HDAC inhibitors to protect neurons from oxygen/glucose deprivation. Two human isoforms are produced by alternative initiation.

Protein type: Nuclear receptor co-regulator; Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; Actin-binding

Chromosomal Location of Human Ortholog: 9q33

Cellular Component: ruffle; focal adhesion; protein complex; perinuclear region of cytoplasm; lamellipodium; extracellular region; cytosol; actin cytoskeleton

Molecular Function: protein binding; calcium ion binding; actin binding

Biological Process: regulation of cell adhesion; actin filament severing; phosphoinositide-mediated signaling; apoptosis; actin nucleation; vesicle-mediated transport; response to ethanol; actin filament polymerization; tissue regeneration; response to folic acid; oligodendrocyte development; barbed-end actin filament capping; cell structure disassembly during apoptosis; aging

Disease: Amyloidosis, Finnish Type