Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Gamma-glutamylcysteine synthetase (gamma-GCS) Standard Curve.)
Human Gamma-Glutamylcysteine Synthetase (gamma-GCS) ELISA Kit | gamma-GCS elisa kit
Human Gamma-Glutamylcysteine Synthetase (gamma-GCS) ELISA Kit
Reactivity
Human
Synonyms
Gamma-Glutamylcysteine Synthetase (gamma-GCS); Human Gamma-Glutamylcysteine Synthetase (gamma-GCS) ELISA Kit; gamma-GCS; glutamate-cysteine ligase; gamma-glutamylcysteine synthetase; gamma-GCS elisa kit
Reactivity
Human
Specificity
Human Gamma-glutamylcysteine synthetase (gamma-GCS) ELISA Kit has high sensitivity and excellent specificity for detection of Human gamma-GCS. No significant cross-reactivity or interference between Human gamma-GCS and analogues was observed.
Sequence Length
688
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Gamma-glutamylcysteine synthetase (gamma-GCS) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Gamma-glutamylcysteine synthetase (gamma-GCS) Standard Curve.)
Related Product Information for gamma-GCS elisa kit
Description: This Human Gamma-glutamylcysteine synthetase (gamma-GCS) ELISA Kit employs a two-site sandwich ELISA to quantitate gamma-GCS in samples. An antibody specific for gamma-GCS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any gamma-GCS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for gamma-GCS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of gamma-GCS bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.It catalyses the ATP-dependent condensation of cysteine and glutamate to form the dipeptide gamma-glutamylcysteine. The peptide bond in this peptide product is between the carboxylate group of the glutamate and the amino group of the cysteine.The product of the GCL-mediated condensation reaction of L-glutamate and L-cysteine is gamma-glutamylcysteine, which is readily condensed with glycine, by glutathione synthetase, to form glutathione.
Background: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.It catalyses the ATP-dependent condensation of cysteine and glutamate to form the dipeptide gamma-glutamylcysteine. The peptide bond in this peptide product is between the carboxylate group of the glutamate and the amino group of the cysteine.The product of the GCL-mediated condensation reaction of L-glutamate and L-cysteine is gamma-glutamylcysteine, which is readily condensed with glycine, by glutathione synthetase, to form glutathione.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
gamma-glutamylcysteine synthetase
Similar Products
Product Notes
The Human gamma-GCS (Catalog #AAA9716183) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716183 ELISA Kit recognizes Human gamma-GCS. It is sometimes possible for the material contained within the vial of "Gamma-Glutamylcysteine Synthetase (gamma-GCS), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
