Human GABPA (GA-binding protein alpha chain) ELISA Kit | GABPA elisa kit

Human GABPA (GA-binding protein alpha chain) ELISA Kit

Cat. Num. AAA7606701

• Gene Names: GABPA; NFT2; NRF2; NRF2A; E4TF1A; E4TF1-60; RCH04A07
• Reactivity: Human
• Synonyms: GABPA (GA-binding protein alpha chain); Human GABPA (GA-binding protein alpha chain) ELISA Kit; NRF2; Nuclear respiratory factor 2; GABPA elisa kit

• Reactivity: Human
• Sequence Length: 454

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78.125-5000pg/ml
• Sensitivity: 46.875pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GABPA elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- GABPA antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-GABPA antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the GABPA amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of GABPA can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 729553
• NCBI GeneID: 2551
• UniProt Accession #: Q06546; Q12939
• Molecular Weight: 51,295 Da
• NCBI Official Full Name: GA-binding protein alpha chain
• NCBI Official Synonym Full Names: GA binding protein transcription factor alpha subunit
• NCBI Official Symbol: GABPA
• NCBI Official Synonym Symbols: NFT2; NRF2; NRF2A; E4TF1A; E4TF1-60; RCH04A07
• NCBI Protein Information: GA-binding protein alpha chain
• UniProt Protein Name: GA-binding protein alpha chain
• Protein Family: GA-binding protein
• UniProt Gene Name: GABPA
• UniProt Synonym Gene Names: E4TF1A; GABP subunit alpha

NCBI Description

This gene encodes one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Oct 2010]

Uniprot Description

GABPA: Transcription factor capable of interacting with purine rich repeats (GA repeats). Necessary for the expression of the Adenovirus E4 gene. Belongs to the ETS family.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 21q21.3

Cellular Component: nuclear chromatin; nucleoplasm; nucleus

Molecular Function: DNA binding; protein binding; protein heterodimerization activity; transcription coactivator activity; transcription factor activity

Biological Process: cell differentiation; mitochondrion organization and biogenesis; positive regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter