Human G Protein Alpha 11 (GNa11) ELISA Kit | GNa11 elisa kit

Human G Protein Alpha 11 (GNa11) ELISA Kit

Cat. Num. AAA2705686

• Gene Names: GNA11; FBH; FBH2; FHH2; HHC2; GNA-11; HYPOC2
• Reactivity: Human
• Synonyms: G Protein Alpha 11 (GNa11); Human G Protein Alpha 11 (GNa11) ELISA Kit; GA11; Guanine Nucleotide-Binding Protein Subunit Alpha-11; Guanine nucleotide-binding protein G(y) subunit alpha; GNa11 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of G Protein Alpha 11 (GNa11).; No significant cross-reactivity or interference between G Protein Alpha 11 (GNa11) and analogues was observed.
• Sequence Length: 359

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.059ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level G Protein Alpha 11 (GNa11) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level G Protein Alpha 11 (GNa11) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GNa11 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to G Protein Alpha 11 (GNa11). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to G Protein Alpha 11 (GNa11). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain G Protein Alpha 11 (GNa11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of G Protein Alpha 11 (GNa11) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 3289982
• NCBI GeneID: 2767
• UniProt Accession #: P29992; O15109; Q14350; Q6IB00
• Molecular Weight: 42,123 Da
• NCBI Official Full Name: GB11_HUMAN
• NCBI Official Synonym Full Names: G protein subunit alpha 11
• NCBI Official Symbol: GNA11
• NCBI Official Synonym Symbols: FBH; FBH2; FHH2; HHC2; GNA-11; HYPOC2
• NCBI Protein Information: guanine nucleotide-binding protein subunit alpha-11
• UniProt Protein Name: Guanine nucleotide-binding protein subunit alpha-11
• Protein Family: Guanine nucleotide-binding protein
• UniProt Gene Name: GNA11
• UniProt Synonym Gene Names: GA11; G alpha-11; G-protein subunit alpha-11

NCBI Description

The protein encoded by this gene belongs to the family of guanine nucleotide-binding proteins (G proteins), which function as modulators or transducers in various transmembrane signaling systems. G proteins are composed of 3 units: alpha, beta and gamma. This gene encodes one of the alpha subunits (subunit alpha-11). Mutations in this gene have been associated with hypocalciuric hypercalcemia type II (HHC2) and hypocalcemia dominant 2 (HYPOC2). Patients with HHC2 and HYPOC2 exhibit decreased or increased sensitivity, respectively, to changes in extracellular calcium concentrations. [provided by RefSeq, Dec 2013]

Uniprot Description

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Acts as an activator of phospholipase C.