Typical Testing Data/Standard Curve (for reference only)
Human Formimidoyltransferase cyclodeaminase (FTCD) ELISA Kit | FTCD elisa kit
Human Formimidoyltransferase cyclodeaminase (FTCD) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: FTCD ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-FTCD antibody and an FTCD-HRP conjugate. The assay sample and buffer are incubated together with FTCD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FTCD concentration since FTCD from samples and FTCD-HRP conjugate compete for the anti-FTCD antibody binding site. Since the number of sites is limited, as more sites are occupied by FTCD from the sample, fewer sites are left to bind FTCD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FTCD concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Mutations in this gene are associated with glutamate formiminotransferase deficiency. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Dec 2009]
Uniprot Description
FTCD: Folate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool. Defects in FTCD are the cause of glutamate formiminotransferase deficiency (FIGLU-URIA); also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Cofactor and Vitamin Metabolism - one carbon pool by folate; Lyase; EC 4.3.1.4; Amino Acid Metabolism - histidine; EC 2.1.2.5; Methyltransferase
Chromosomal Location of Human Ortholog: 21q22.3
Cellular Component: centriole; Golgi apparatus; cytoplasm; cytosol
Molecular Function: formimidoyltetrahydrofolate cyclodeaminase activity; glutamate formimidoyltransferase activity; folic acid binding
Biological Process: folic acid and derivative metabolic process; cytoskeleton organization and biogenesis; histidine catabolic process to glutamate and formamide; histidine catabolic process; histidine catabolic process to glutamate and formate
Disease: Formiminotransferase Deficiency
Research Articles on FTCD
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Product Notes
The Human FTCD ftcd (Catalog #AAA7200718) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7200718 ELISA Kit recognizes Human FTCD. It is sometimes possible for the material contained within the vial of "Formimidoyltransferase cyclodeaminase (FTCD), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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