Human Ficolin-1 ELISA Kit | FCN1 elisa kit

Ficolin-1, Human, ELISA Kit

Cat. Num. AAA584531

• Gene Names: FCN1; FCNM
• Reactivity: Human
• Synonyms: Ficolin-1; Human; ELISA Kit; M-ficolin; ficolin/P35-related; FCN1 elisa kit

• Reactivity: Human
• Sequence Length: 326

• Detection Level: 3.1 ng/ml
• Linearity: The linearity of the assay was determined by serially diluting plasma and serum samples containing human Ficolin-1. The diluted samples were measured in the assay.
• Recovery: Normal human blood samples (plasma) containing baseline levels of human Ficolin-1, were spiked with human Ficolin-1, in concentrations of 2 and 0.2ug/ml. Samples with and without human Ficolin-1, were incubated for 1 hour at 37 degree C. Samples were measured using the ELISA. Recovery values for human Ficolin-1, ranged between 93.9% and 97.4%.
• Standard Range: 3.1 to 200 ng/ml
• Working Volume: 100 ul/well
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for FCN1 elisa kit

Product Description: Ficolins are a family of pattern recognition molecules containing both a collagen-like domain and a fibrinogen-like domain that are capable of activating the complement system, through association with MASPs, on the surface of microorganisms. In human, three Ficolins are known: Ficolin-1 (M-ficolin), Ficolin-2 (L-ficolin), and Ficolin-3 (H-ficolin). Ficolin-1, is mainly expressed by monocytes, granulocytes and lung cells and is present locally in areas of inflammation. However, it was recently also found in serum suggesting that Ficolin-1 also has a role in systemic immunity like Ficolin-2 and Ficolin-3. Functional analysis of Ficolin-1 has demonstrated that it binds specifically to capsular polysaccharides of some microorganisms. Furthermore, it was shown that Ficolin-1 facilitates the uptake of E. coli by a leukemic monocyte lymphoma cell line, suggesting that it functions as a phagocytic receptor on the surface of circulating monocytes. Low cord blood concentration of Ficolin-1 has been associated with higher necrotizing enterocolitis-associated fatality in premature infants and polymorphisms in the Ficolin-1 gene are associated with the development of rheumatoid arthritis and susceptibility to leprosy.
Principle of the assay: The human Ficolin-1 ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with antibodies recognizing human Ficolin-1. Biotinylated tracer antibody will bind to the captured human M-ficolin. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human Ficolin-1 standards (log).

NCBI and Uniprot Product Information

• NCBI GI #: 8051584
• NCBI GeneID: 2219
• NCBI Accession #: NP_001994.2
• NCBI GenBank Nucleotide #: NM_002003.3
• Molecular Weight: 35,078 Da
• NCBI Official Full Name: ficolin-1
• NCBI Official Synonym Full Names: ficolin (collagen/fibrinogen domain containing) 1
• NCBI Official Symbol: FCN1
• NCBI Official Synonym Symbols: FCNM
• NCBI Protein Information: ficolin-1; M-ficolin; ficolin-A; ficolin-alpha; collagen/fibrinogen domain-containing protein 1; ficolin (collagen/fibrinogen domain-containing) 1
• UniProt Protein Name: Ficolin-1
• Protein Family: Ficolin
• UniProt Gene Name: FCN1
• UniProt Synonym Gene Names: FCNM
• UniProt Entry Name: FCN1_HUMAN