Human Fibronectin (FN) ELISA Kit | FN elisa kit

Human Fibronectin (FN) High Sensitive ELISA Kit

Cat. Num. AAA2707963

• Reactivity: Human
• Applications: ELISA
• Synonyms: Fibronectin (FN); Human Fibronectin (FN) High Sensitive ELISA Kit; Fibronectin; FN1; CIG; FINC; LETS; MSF; GFND2; Anastellin; Migration-Stimulating Factor; Cold-Insoluble Globulin; Large; External; Transformation-Sensitive Protein; FN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of High Sensitive Fibronectin (FN).; No significant cross-reactivity or interference between High Sensitive Fibronectin (FN) and analogues was observed.
• Sequence Length: 2355

• Applicable Applications for FN elisa kit: ELISA (EIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.053ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Fibronectin (FN) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Fibronectin (FN) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Fibronectin (FN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Fibronectin (FN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Fibronectin (FN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Fibronectin (FN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 16933542
• NCBI Accession #: NP_002017.1
• NCBI GenBank Nucleotide #: NP_002017.1
• UniProt Accession #: P02751
• NCBI Official Full Name: fibronectin isoform 3 preproprotein
• UniProt Protein Name: Fibronectin
• Protein Family: Fibronectin
• UniProt Gene Name: FN1
• UniProt Synonym Gene Names: FN; FN; CIG
• UniProt Entry Name: FINC_HUMAN

Uniprot Description

FN1: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Defects in FN1 are the cause of glomerulopathy with fibronectin deposits type 2 (GFND2); also known as familial glomerular nephritis with fibronectin deposits or fibronectin glomerulopathy. GFND is a genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life. 15 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 2q34

Cellular Component: extracellular matrix; extracellular space; apical plasma membrane; fibrinogen complex; extracellular region; ER-Golgi intermediate compartment; basal lamina

Molecular Function: heparin binding; collagen binding; integrin binding; protein binding; protease activator activity; protease binding

Biological Process: integrin activation; platelet activation; extracellular matrix organization and biogenesis; positive regulation of axon extension; extracellular matrix disassembly; regulation of cell shape; platelet degranulation; acute-phase response; calcium-independent cell-matrix adhesion; cell-substrate junction assembly; response to wounding; peptide cross-linking; angiogenesis; cell adhesion; blood coagulation; leukocyte migration

Disease: Glomerulopathy With Fibronectin Deposits 2; Plasma Fibronectin Deficiency