Typical Testing Data/Standard Curve (for reference only)
Human FGL1 ELISA Kit | FGL1 elisa kit
Human FGL1 (Fibrinogen Like Protein 1) ELISA Kit
Gene Names
FGL1; HFREP1; HP-041; LFIRE1; LFIRE-1
Reactivity
Human
Synonyms
FGL1; Human FGL1 (Fibrinogen Like Protein 1) ELISA Kit; FGL1 elisa kit
Reactivity
Human
Specificity
This kit recognizes Human FGL1 in samples. No significant cross-reactivity or interference between Human FGL1 and analogues was observed.
Sequence Length
312
Samples
Serum, plasma and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.16-10ng/mL
Sensitivity
0.1ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human FGL1 were tested 20 times on one plate, respectively.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human FGL1 were tested on 3 different plates, 20 replicates in each plate.
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for FGL1 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human FGL1 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FGL1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FGL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human FGL1. You can calculate the concentration of Human FGL1 in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FGL1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FGL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human FGL1. You can calculate the concentration of Human FGL1 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36,379 Da
NCBI Official Full Name
fibrinogen-like protein 1
NCBI Official Synonym Full Names
fibrinogen-like 1
NCBI Official Symbol
FGL1
NCBI Official Synonym Symbols
HFREP1; HP-041; LFIRE1; LFIRE-1
NCBI Protein Information
fibrinogen-like protein 1; hepassocin; hepatocellular carcinoma-related sequence; hepatocyte-derived fibrinogen-related protein 1; liver fibrinogen-related protein 1
UniProt Protein Name
Fibrinogen-like protein 1
Protein Family
UniProt Gene Name
FGL1
UniProt Synonym Gene Names
HFREP1; HFREP-1; LFIRE-1
UniProt Entry Name
FGL1_HUMAN
NCBI Description
Fibrinogen-like 1 is a member of the fibrinogen family. This protein is homologous to the carboxy terminus of the fibrinogen beta- and gamma- subunits which contains the four conserved cysteines of fibrinogens and fibrinogen related proteins. However, this protein lacks the platelet-binding site, cross-linking region and a thrombin-sensitive site which are necessary for fibrin clot formation. This protein may play a role in the development of hepatocellular carcinomas. Four alternatively spliced transcript variants encoding the same protein exist for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
FGL1: Has hepatocyte mitogenic activity.
Protein type: Secreted, signal peptide; Extracellular matrix; Secreted
Chromosomal Location of Human Ortholog: 8p22
Cellular Component: fibrinogen complex
Biological Process: cholesterol metabolic process
Research Articles on FGL1
Similar Products
Product Notes
The Human FGL1 fgl1 (Catalog #AAA2514944) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2514944 ELISA Kit recognizes Human FGL1. It is sometimes possible for the material contained within the vial of "FGL1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
