Human FABP4 ELISA Kit | FABP4 elisa kit

Human FABP4 PicoKine ELISA Kit

Cat. Num. AAA178529

• Gene Names: FABP4; aP2; ALBP; AFABP; A-FABP; HEL-S-104
• Reactivity: Human
• Synonyms: FABP4; Human FABP4 PicoKine ELISA Kit; 422/aP2; A-FABP; AFABP; adipocyte; ALBP; ALBP/Ap2; aP2; HEL S 104; Lbpl; P15; Protein 422; P15090; Fatty acid-binding protein; fatty acid binding protein 4; FABP4 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human FABP4
• Sequence Length: 132

• Samples: Cell culture supernates, serum, plasma(heparin, EDTA) and cell lysates
• Detection Range: 156pg/ml-10000pg/ml
• Sensitivity: <15pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FABP4 elisa kit

Principle of the Assay: The Picokine™ Human FABP4 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human FABP4 with a 96-well strip plate that is pre-coated with antibody specific for FABP4. The detection antibody is a biotinylated antibody specific for FABP4. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human FABP4 with immunogen: Expression system for standard: E.coli; Immunogen sequence: M1-A132. The kit is analytically validated with ready to use reagents. To measure Human FABP4, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human FABP4 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human FABP4 in the sample.!!Background/Introduction: FABP4, also known as aP2 (adipocyte Protein 2), encodes the fatty acid binding protein found in adipocytes. The FABP4 gene is mapped to 8q21. FABP4 functions as a cytosolic lipid chaperone in macrophages and is involved in regulating macrophage endoplasmic reticulum (ER) stress. Aditionally, FABP4 protein is expressed in HBEs and is strongly upregulated by both IL4 and IL13 and downregulated by IFN-gamma, suggesting a role in allergic airway inflammation.

NCBI and Uniprot Product Information

• NCBI GI #: 4557579
• NCBI GeneID: 2167
• NCBI Accession #: NP_001433.1
• NCBI GenBank Nucleotide #: NM_001442.2
• UniProt Accession #: P15090; Q6IBA1
• Molecular Weight: 14,719 Da
• NCBI Official Full Name: fatty acid-binding protein, adipocyte
• NCBI Official Synonym Full Names: fatty acid binding protein 4
• NCBI Official Symbol: FABP4
• NCBI Official Synonym Symbols: aP2; ALBP; AFABP; A-FABP; HEL-S-104
• NCBI Protein Information: fatty acid-binding protein, adipocyte
• UniProt Protein Name: Fatty acid-binding protein, adipocyte
• Protein Family: Fatty acid-binding protein
• UniProt Gene Name: FABP4
• UniProt Synonym Gene Names: ALBP; A-FABP; AFABP
• UniProt Entry Name: FABP4_HUMAN

NCBI Description

FABP4 encodes the fatty acid binding protein found in adipocytes. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. [provided by RefSeq, Jul 2008]

Uniprot Description

FABP4: a lipid transport protein that is the predominant fatty acid binding protein found in adipocytes. Also expressed in macrophages. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus. Homodimer. Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. Interacts with PPARG. Monomer. FABP4 knockout mice fed a high-fat and high-calorie diet become obese but develop neither insulin resistance nor diabetes, suggesting that this protein might be a link between obesity and insulin resistance and diabetes. Mice deficient in both FABP4 and ApoE show protection against atherosclerosis when compared with mice deficient only in ApoE. Mice carrying a FABP4 genetic variant exhibit both reduced FABP4 expression and a reduced potential for developing type 2 diabetes and coronary heart disease. A related study in humans indicated a similar pattern, suggesting that FABP4 may be a potential therapeutic target in the treatment of these disorders. Belongs to the calycin superfamily,fatty-acid binding protein (FABP) family.

Protein type: Lipid-binding

Chromosomal Location of Human Ortholog: 8q21

Cellular Component: cytoplasm; cytosol; lipid particle

Biological Process: triacylglycerol catabolic process