Human ETV4 (ETS translocation variant 4) ELISA Kit | ETV4 elisa kit

Human ETV4 (ETS translocation variant 4) ELISA Kit

Cat. Num. AAA7608218

• Gene Names: ETV4; E1AF; PEA3; E1A-F; PEAS3
• Reactivity: Human
• Synonyms: ETV4 (ETS translocation variant 4); Human ETV4 (ETS translocation variant 4) ELISA Kit; ETS translocation variant 4; Adenovirus E1A enhancer-binding protein; E1A-F; Polyomavirus enhancer activator 3 homolog; Protein PEA3; ETV4; E1AF; PEA3; ETV4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ETV4. No significant cross-reactivity or interference between ETV4 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.125-5000pg/ml
• Sensitivity: 46.875pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for est. ~6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ETV4 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 118918421
• NCBI GeneID: 2118
• NCBI Accession #: NP_001073143.1
• NCBI GenBank Nucleotide #: NM_001079675.2
• UniProt Accession #: P43268; Q96AW9; A8K314; B7Z5J3; B7Z9J6
• Molecular Weight: 53,938 Da
• NCBI Official Full Name: ETS translocation variant 4 isoform 1
• NCBI Official Synonym Full Names: ets variant 4
• NCBI Official Symbol: ETV4
• NCBI Official Synonym Symbols: E1AF; PEA3; E1A-F; PEAS3
• NCBI Protein Information: ETS translocation variant 4; adenovirus E1A enhancer-binding protein; polyomavirus enhancer activator 3 homolog; EWS protein/E1A enhancer binding protein chimera; ets variant gene 4 (E1A enhancer-binding protein, E1AF)
• UniProt Protein Name: ETS translocation variant 4
• Protein Family: ETS translocation
• UniProt Gene Name: ETV4
• UniProt Synonym Gene Names: E1AF; PEA3; Protein PEA3
• UniProt Entry Name: ETV4_HUMAN

Uniprot Description

ETV4: Transcriptional activator that binds to the enhancer of the adenovirus E1A gene; the core-binding sequence is 5'[AC]GGA[AT]GT-3'. Belongs to the ETS family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: nucleolus

Molecular Function: protein binding

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; motor axon guidance; positive regulation of transcription from RNA polymerase II promoter; stem cell differentiation; cell differentiation; negative regulation of mammary gland epithelial cell proliferation