Human ENA Screen ELISA Kit | ENA elisa kit
ENA Screen ELISA
Reactivity
Human
Synonyms
ENA Screen; ENA Screen ELISA; ENA elisa kit
Reactivity
Human
Specificity
The microplate is coated with a mixture of the antigens SS-A (Ro), SS-B (La), Sm, RNP/Sm, Scl 70 and Jo-1, highly purified by affinity chromatography. The ENAscreen test is specific only for autoantibodies directed to these antigens. No crossreactivities have been observed.
Samples
Human serum or plasma
Intended Uses
ENAscreen is an indirect solid phase enzyme immunoassay (ELISA) for the qualitative screening of IgG class autoantibodies against extractable nuclear antigens (ENA) in human serum or plasma.
Preparation and Storage
2 - 8 degree C
Related Product Information for ENA elisa kit
Principle of the assay: A mixture of extractable nuclear antigens (SS-A (Ro), SS-B (La), Sm, RNP/Sm, Scl 70 and Jo-1) are bound to microwells. Antibodies against these antigens, if present in diluted serum or plasma, bind to the respective antigens. Washing of the microwells removes unspecific serum and plasma components. Horseradish peroxidase (HRP) conjugated anti-human IgG immunologically detects the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow end-product. The intensity of this yellow color is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG antibodies present in the original sample.
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Product Notes
The Human ENA (Catalog #AAA494669-ORG 506) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA494669-ORG 506 ELISA Kit recognizes Human ENA. It is sometimes possible for the material contained within the vial of "ENA Screen, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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