Human Elastase 4 (ELA4) ELISA Kit | ELA4 elisa kit

Human Elastase 4 (ELA4) ELISA Kit

Cat. Num. AAA455652

• Gene Names: CTRC; CLCR; ELA4
• Reactivity: Human
• Synonyms: Elastase 4 (ELA4); Human Elastase 4 (ELA4) ELISA Kit; CLCR; CTRC; Chymotrypsin C; Caldecrin; ELA4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ELA4. No significant cross-reactivity or interference between ELA4 and analogues was observed.
• Sequence Length: 268

• Samples: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 3.125-200ng/mL
• Sensitivity: 1.35ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ELA4 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ELA4 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ELA4 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ELA4 in human serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ELA4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ELA4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ELA4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ELA4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 62526043
• NCBI GeneID: 11330
• NCBI Accession #: NP_009203.2
• NCBI GenBank Nucleotide #: NM_007272.2
• UniProt Accession #: Q99895; O00765; Q9NUH5; A8K082
• Molecular Weight: 29,484 Da
• NCBI Official Full Name: chymotrypsin-C preproprotein
• NCBI Official Synonym Full Names: chymotrypsin C
• NCBI Official Symbol: CTRC
• NCBI Official Synonym Symbols: CLCR; ELA4
• NCBI Protein Information: chymotrypsin-C
• UniProt Protein Name: Chymotrypsin-C
• UniProt Gene Name: CTRC
• UniProt Synonym Gene Names: CLCR

NCBI Description

This gene encodes a member of the peptidase S1 family. The encoded protein is a serum calcium-decreasing factor that has chymotrypsin-like protease activity. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Regulates activation and degradation of trypsinogens and procarboxypeptidases by targeting specific cleavage sites within their zymogen precursors. Has chymotrypsin-type protease activity and hypocalcemic activity.