Human E3 ubiquitin-protein ligase MARCH11 (MARCH11) ELISA Kit | MARCH11 elisa kit

Human E3 ubiquitin-protein ligase MARCH11 (MARCH11) ELISA Kit

Cat. Num. AAA2800254

• Gene Names: MARCH11; RNF226; MARCH-XI
• Reactivity: Human
• Synonyms: E3 ubiquitin-protein ligase MARCH11 (MARCH11); Human E3 ubiquitin-protein ligase MARCH11 (MARCH11) ELISA Kit; MARCH-XI; MARCH11 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human MARCH11. No significant cross-reactivity or interference between Human MARCH11 and analogues was observed.
• Sequence Length: 402

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for MARCH11 elisa kit

This assay employs a two-site sandwich ELISA to quantitate MARCH11 in samples. An antibody specific for MARCH11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MARCH11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MARCH11 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MARCH11 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 156523244
• NCBI GeneID: 441061
• NCBI Accession #: NP_001096032.1
• NCBI GenBank Nucleotide #: NM_001102562.2
• UniProt Accession #: A6NNE9; A7E2S6
• Molecular Weight: 18,226 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase MARCH11
• NCBI Official Synonym Full Names: membrane associated ring-CH-type finger 11
• NCBI Official Symbol: MARCH11
• NCBI Official Synonym Symbols: RNF226; MARCH-XI
• NCBI Protein Information: E3 ubiquitin-protein ligase MARCH11
• UniProt Protein Name: E3 ubiquitin-protein ligase MARCH11
• UniProt Gene Name: MARCH11
• UniProt Synonym Gene Names: MARCH-XI

NCBI Description

MARCH11 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). These enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their intracellular transport. March11 appears to have a role in ubiquitin-mediated protein sorting in the trans-Golgi network (TGN)-multivesicular body (MVB) transport pathway (Morokuma et al., 2007 [PubMed 17604280]).[supplied by OMIM, Apr 2010]

Uniprot Description

E3 ubiquitin-protein ligase that mediates polyubiquitination of CD4. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. May play a role in ubuquitin-dependent protein sorting in developmenting spermatids.