Human E-Cadherin, E-Cad ELISA Kit | E-Cad elisa kit

Human E-Cadherin, E-Cad ELISA Kit

Cat. Num. AAA164809

• Gene Names: CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324
• Reactivity: Human
• Synonyms: E-Cadherin; E-Cad; Human E-Cadherin; E-Cad ELISA Kit; E-Cad elisa kit

• Reactivity: Human
• Sequence Length: 827

• Samples: Serum, plasma, cell culture supernates, ascites, tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 1-300ng/ml
• Sensitivity: 0.52ng/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for E-Cad elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of Human E-Cadherin (also known as E-Cad) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human E-Cad antibody. E-Cad present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human E-Cad Antibody is added and binds to E-Cad in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated E-Cad antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human E-Cad. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 1617084
• NCBI GeneID: 999
• UniProt Accession #: P12830; Q13799; Q14216; Q15855; Q16194; Q4PJ14; Q9UII8; A8K1U7
• Molecular Weight: 90,942 Da
• NCBI Official Full Name: E-cadherin, partial
• NCBI Official Synonym Full Names: cadherin 1
• NCBI Official Symbol: CDH1
• NCBI Official Synonym Symbols: UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324
• NCBI Protein Information: cadherin-1
• UniProt Protein Name: Cadherin-1
• UniProt Gene Name: CDH1
• UniProt Synonym Gene Names: CDHE; UVO; E-cadherin

NCBI Description

This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015]

Uniprot Description

Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.