Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Dynein heavy chain 11, axonemal (DNAH11) Standard Curve.)
Human Dynein Heavy Chain 11, Axonemal (DNAH11) ELISA Kit | DNAH11 elisa kit
Human Dynein Heavy Chain 11, Axonemal (DNAH11) ELISA Kit
Gene Names
DNAH11; CILD7; DNHBL; DPL11; DNAHBL; DNAHC11
Reactivity
Human
Synonyms
Dynein Heavy Chain 11; Axonemal (DNAH11); Human Dynein Heavy Chain 11; Axonemal (DNAH11) ELISA Kit; DNAH11; CILD7; DNAHBL; DNAHC11; DNHBL; DPL11; FLJ30095; FLJ37699; dynein; axonemal; heavy polypeptide 11; ciliary; heavy chain 11; heavy chain beta-like; DNAH11 elisa kit
Reactivity
Human
Specificity
Human Dynein heavy chain 11, axonemal (DNAH11) ELISA Kit has high sensitivity and excellent specificity for detection of Human DNAH11. No significant cross-reactivity or interference between Human DNAH11 and analogues was observed.
Sequence Length
4516
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Dynein heavy chain 11, axonemal (DNAH11) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Dynein heavy chain 11, axonemal (DNAH11) Standard Curve.)
Related Product Information for DNAH11 elisa kit
Description: This Human Dynein heavy chain 11, axonemal (DNAH11) ELISA Kit employs a two-site sandwich ELISA to quantitate DNAH11 in samples. An antibody specific for DNAH11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DNAH11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DNAH11 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DNAH11 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: DNAH11 encodes a member of the dynein heavy chain family. It is a microtubule-dependent motor ATPase and has been reported to be involved in the movement of respiratory cilia. Mutations in this gene have been implicated in causing Kartagener Syndrome (a combination of situs inversus totalis and Primary Ciliary Dyskinesia (PCD), also called Immotile Cilia Syndrome 1 (ICS1)) and male sterility. The axonemal dyneins, found in cilia and flagella, are components of the outer and inner dynein arms attached to the peripheral microtubule doublets. DNAHC11 belongs to the putative axonemal outer dynein arm DHC group and is homologous to rat DLP11. RT-PCR assays indicated that DNAHC11 is expressed primarily in trachea and testis, 2 tissues containing axonemal structures.
Background: DNAH11 encodes a member of the dynein heavy chain family. It is a microtubule-dependent motor ATPase and has been reported to be involved in the movement of respiratory cilia. Mutations in this gene have been implicated in causing Kartagener Syndrome (a combination of situs inversus totalis and Primary Ciliary Dyskinesia (PCD), also called Immotile Cilia Syndrome 1 (ICS1)) and male sterility. The axonemal dyneins, found in cilia and flagella, are components of the outer and inner dynein arms attached to the peripheral microtubule doublets. DNAHC11 belongs to the putative axonemal outer dynein arm DHC group and is homologous to rat DLP11. RT-PCR assays indicated that DNAHC11 is expressed primarily in trachea and testis, 2 tissues containing axonemal structures.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
dynein heavy chain 11, axonemal
NCBI Official Synonym Full Names
dynein axonemal heavy chain 11
NCBI Official Symbol
DNAH11
NCBI Official Synonym Symbols
CILD7; DNHBL; DPL11; DNAHBL; DNAHC11
NCBI Protein Information
dynein heavy chain 11, axonemal
UniProt Protein Name
Dynein heavy chain 11, axonemal
Protein Family
UniProt Gene Name
DNAH11
NCBI Description
This gene encodes a ciliary outer dynein arm protein and is a member of the dynein heavy chain family. It is a microtubule-dependent motor ATPase and has been reported to be involved in the movement of respiratory cilia. Mutations in this gene have been implicated in causing Kartagener Syndrome (a combination of situs inversus totalis and Primary Ciliary Dyskinesia (PCD), also called Immotile Cilia Syndrome 1 (ICS1)) and male sterility. [provided by RefSeq, Mar 2013]
Uniprot Description
Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP.
Research Articles on DNAH11
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Product Notes
The Human DNAH11 dnah11 (Catalog #AAA9715665) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715665 ELISA Kit recognizes Human DNAH11. It is sometimes possible for the material contained within the vial of "Dynein Heavy Chain 11, Axonemal (DNAH11), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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