Human Dopamine Transporter (DAT) ELISA Kit | DAT elisa kit

Human Dopamine Transporter (DAT) ELISA Kit

Cat. Num. AAA2019396

• Gene Names: SLC6A3; DAT; DAT1; PKDYS
• Reactivity: Human
• Synonyms: Dopamine Transporter (DAT); Human Dopamine Transporter (DAT) ELISA Kit; SLC6A3; DAT1; Sodium-dependent dopamine transporter; Dopamine Active Transporter; SLC6-A3; Solute Carrier Family 6(neurotransmitter Transporter; Dopamine); Member 3; DAT elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Dopamine Transporter (DAT).; No significant cross-reactivity or interference between Dopamine Transporter (DAT) and analogues was observed.
• Sequence Length: 620

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dopamine Transporter (DAT) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dopamine Transporter (DAT) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DAT elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dopamine Transporter (DAT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dopamine Transporter (DAT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dopamine Transporter (DAT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dopamine Transporter (DAT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for DAT elisa kit

Signal transduction; Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 7108463
• NCBI GeneID: 6531
• UniProt Accession #: Q01959; Q14996; A2RUN4
• Molecular Weight: 68,495 Da
• NCBI Official Full Name: dopamine transporter
• NCBI Official Synonym Full Names: solute carrier family 6 (neurotransmitter transporter), member 3
• NCBI Official Symbol: SLC6A3
• NCBI Official Synonym Symbols: DAT; DAT1; PKDYS
• NCBI Protein Information: sodium-dependent dopamine transporter
• UniProt Protein Name: Sodium-dependent dopamine transporter
• Protein Family: D-alanine aminotransferase
• UniProt Gene Name: SLC6A3
• UniProt Synonym Gene Names: DAT1; DA transporter; DAT
• UniProt Entry Name: SC6A3_HUMAN

NCBI Description

This gene encodes a dopamine transporter which is a member of the sodium- and chloride-dependent neurotransmitter transporter family. The 3' UTR of this gene contains a 40 bp tandem repeat, referred to as a variable number tandem repeat or VNTR, which can be present in 3 to 11 copies. Variation in the number of repeats is associated with idiopathic epilepsy, attention-deficit hyperactivity disorder, dependence on alcohol and cocaine, susceptibility to Parkinson disease and protection against nicotine dependence.[provided by RefSeq, Nov 2009]

Uniprot Description

DAT: Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals. Defects in SLC6A3 are the cause of dystonia-parkinsonism infantile (DYTPRI). It is a neurodegenerative disorder characterized by infantile onset of parkinsonism and dystonia. Other neurologic features include global developmental delay, bradikinesia and pyramidal tract signs. Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A3 subfamily.

Protein type: Membrane protein, multi-pass; Transporter, SLC family; Membrane protein, integral; Transporter

Chromosomal Location of Human Ortholog: 5p15.3

Cellular Component: cell soma; axon; integral to plasma membrane; cytoplasm; integral to membrane; plasma membrane

Molecular Function: protein binding; protease binding; monoamine transmembrane transporter activity; dopamine binding; protein complex binding; protein N-terminus binding; drug binding; dopamine:sodium symporter activity; dopamine transmembrane transporter activity; receptor binding

Biological Process: lactation; response to drug; response to nicotine; dopamine transport; response to cAMP; dopamine catabolic process; prepulse inhibition; monoamine transport; sensory perception of smell; regulation of dopamine metabolic process; locomotory behavior; positive regulation of multicellular organism growth; dopamine biosynthetic process; response to cocaine; synaptic transmission; response to ethanol; neurotransmitter transport; adenohypophysis development; response to iron ion; transmembrane transport; neurotransmitter biosynthetic process; aging

Disease: Parkinsonism-dystonia, Infantile; Tobacco Addiction, Susceptibility To