Typical Testing Data/Standard Curve (for reference only)
Human Dolichyl diphosphooligosaccharide protein glycosyltransferase subunit 2 (RPN2) ELISA Kit | RPN2 elisa kit
Human Dolichyl diphosphooligosaccharide protein glycosyltransferase subunit 2 (RPN2) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: RPN2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-RPN2 antibody and an RPN2-HRP conjugate. The assay sample and buffer are incubated together with RPN2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RPN2 concentration since RPN2 from samples and RPN2-HRP conjugate compete for the anti-RPN2 antibody binding site. Since the number of sites is limited, as more sites are occupied by RPN2 from the sample, fewer sites are left to bind RPN2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RPN2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein is similar in sequence to the yeast oligosaccharyl transferase subunit SWP1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]
Uniprot Description
RPN2: Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. Belongs to the SWP1 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.4.99.18; Transferase; Membrane protein, multi-pass; Membrane protein, integral; Glycan Metabolism - N-glycan biosynthesis
Chromosomal Location of Human Ortholog: 20q12-q13.1
Cellular Component: endoplasmic reticulum membrane; rough endoplasmic reticulum; membrane; endoplasmic reticulum; oligosaccharyl transferase complex; integral to membrane; nucleus
Molecular Function: dolichyl-diphosphooligosaccharide-protein glycotransferase activity; ribosome binding
Biological Process: response to drug; SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translation; protein modification process; gene expression; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification; aging
Research Articles on RPN2
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Product Notes
The Human RPN2 rpn2 (Catalog #AAA7252853) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7252853 ELISA Kit recognizes Human RPN2. It is sometimes possible for the material contained within the vial of "Dolichyl diphosphooligosaccharide protein glycosyltransferase subunit 2 (RPN2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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