Human dolichol kinase ELISA Kit | DOLK elisa kit

Human Dolichol kinase, DOLK ELISA Kit

Cat. Num. AAA2602886

• Gene Names: DOLK; DK; DK1; CDG1M; SEC59; TMEM15
• Reactivity: Human
• Synonyms: dolichol kinase; Human Dolichol kinase; DOLK ELISA Kit; DOLK elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.

• Samples: Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant DOLK concentration.
• Detection Range: 20 ng/ml-0.312 ng/ml
• Sensitivity: Up to 0.06ng/ml.
• Assay Type: Sandwich
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DOLK elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human DOLK monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 7662482
• NCBI GeneID: 22845
• NCBI Accession #: NP_055723.1
• NCBI GenBank Nucleotide #: NM_014908.3
• UniProt Accession #: Q9UPQ8; Q5SRE6
• Molecular Weight: 59,268 Da
• NCBI Official Full Name: dolichol kinase
• NCBI Official Synonym Full Names: dolichol kinase
• NCBI Official Symbol: DOLK
• NCBI Official Synonym Symbols: DK; DK1; CDG1M; SEC59; TMEM15
• NCBI Protein Information: dolichol kinase; SEC59 homolog; dolichol kinase 1; transmembrane protein 15
• UniProt Protein Name: Dolichol kinase
• Protein Family: Dolichol kinase
• UniProt Gene Name: DOLK
• UniProt Synonym Gene Names: KIAA1094; TMEM15; UNQ2422/PRO4980
• UniProt Entry Name: DOLK_HUMAN

NCBI Description

The protein encoded by this gene catalyzes the CTP-mediated phosphorylation of dolichol, and is involved in the synthesis of Dol-P-Man, which is an essential glycosyl carrier lipid for C- and O-mannosylation, N- and O-linked glycosylation of proteins, and for the biosynthesis of glycosyl phosphatidylinositol anchors in endoplasmic reticulum. Mutations in this gene are associated with dolichol kinase deficiency.[provided by RefSeq, Apr 2010]

Uniprot Description

DOLK: Involved in the synthesis of the sugar donor Dol-P-Man which is required in the synthesis of N-linked and O-linked oligosaccharides and for that of GPI anchors. Defects in DOLK are the cause of congenital disorder of glycosylation type 1M (CDG1M); also known as dolichol kinase deficiency. CDGs are a family of severe inherited diseases caused by a defect in glycoprotein biosynthesis. They are characterized by under-glycosylated serum glycoproteins. These multisystem disorders present with a wide variety of clinical features, such as disorders of the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions. CDG1M is a very severe disorder with death occurring in early infancy. Belongs to the polyprenol kinase family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Endoplasmic reticulum; Kinase, other; EC 2.7.1.108

Chromosomal Location of Human Ortholog: 9q34.11

Cellular Component: endoplasmic reticulum membrane; integral to endoplasmic reticulum membrane

Molecular Function: dolichol kinase activity

Biological Process: cellular protein metabolic process; dolichol-linked oligosaccharide biosynthetic process; dolichyl diphosphate biosynthetic process; dolichyl monophosphate biosynthetic process; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification; phosphorylation

Disease: Congenital Disorder Of Glycosylation, Type Im